03L MICROSCOPY.pptx 03L MICROSCOPY.pptx

03L MICROSCOPY.pptx 03L MICROSCOPY.pptx

1. Class notes by Dr. Hira Hameed

2. Microscopy is the technology of making very small things visible to the human eye Milli = one thousandth 10 -3 m Micro = one millionth 10 -6 m Nano = one billionth 10 -9 m Microscopy Class notes by Dr. Hira Hameed

3. Relative Sizes of Objects Class notes by Dr. Hira Hameed

4. “The instrument which presents an enlarged and detailed image of specimen under observation” Key characteristics of a reliable microscope are: Magnification – ability to enlarge objects Resolving power – ability to show detail of two closely situated objects The Microscope Class notes by Dr. Hira Hameed

5. Magnification Vs Resolution Magnification Enhancement of size using objective and ocular lens Ocular lens: 10X Common objective lenses: Scanning (4X), Low power (10X), High dry (40X) Oil immersion (100X) Resolution Ability to distinguish two objects as distinct A general principle of microscopy--- shorter the wavelength of light greater will be resolution Wavelength of light is 550nm > limitation of light microscope. Total Magnification = obj. mag. x ocular mag. Class notes by Dr. Hira Hameed

6. Transmission Electron Microscope Scanning Electron Microscope Compound Light microscope Unaided human eye Magnifying & Resolving Power Resolving Power Magnifying Power Class notes by Dr. Hira Hameed

7. Refers to the use of any kind of microscope that uses visible light to make specimens observable Simple light microscope has only one lens The compound light microscope has more than one lens Objective lens Ocular lens Monocular vs. Binocular Light Microscopy Class notes by Dr. Hira Hameed

8. Simple & Compound Light Microscope Class notes by Dr. Hira Hameed

9. A. Light microscopy 1. Bright-field (light) microscopy 2. Dark-field microscopy 3. Phase-contrast microscopy 4. Fluorescent microscopy 5. Confocal microscopy B. Electron microscopy 1. Transmission EM 2. Scanning EM C. Scanning probe microscopy Types of Microscopes Class notes by Dr. Hira Hameed

10. Class notes by Dr. Hira Hameed

11. Class notes by Dr. Hira Hameed