14. components of genetic variation

Dr. NAVEENKUMAR K.L
Assistant professor
Dept. Genetics and Plant Breeding

1. Components of Genetic variation: Heritability and Genetic advance Dr. NAVEENKUMAR K.L Assistant professor Dept. Genetics and Plant Breeding

2. Components of variation Heritability Types of heritability Genetic advance Environment Genotype x Environment interaction

3. Mendel studied qualitative traits

4. 3:1

5. But many traits are quantitative Quantitative traits are distributed continuously. Activities of many genes usually underlie such traits

6. Discontinuous ( qualitative ) characters

7. Continuous ( quantitative ) characters

8. Inheritance of Quantitative traits Francis Galton – Mendel’s laws of inheritance could not explain the continuous variation. Hugo de Vries – Continuous variations are non-heritable Yule (1906) – Many genes with small and similar effects produce continuous variation.

9. Kernel color in wheat (Nilsson-Ehle 1909) Three loci 1 6 15 20 15 6 1 P F 1 F 2 Experimental Evidence 63:1 Multiple Factor Hypothesis: Characters are governed by many genes with small and cumulative effect Dark Red Medium Dark Red Medium Red Red Medium Light Red Light Red White

10. Quantitative traits Multiple loci (genes) many genes contribute to variation! Influenced by environment Show continuous variation Qualitative traits One or few genes Least influenced by environment Show discrete class Qualitative v/s Quantitative traits

11. BIOMETRICS IN PLANT BREEDING - QUALITATIVE TRAIT A qualitative trait is expressed qualitatively , which means that the phenotype falls into different categories . Qualitative traits can be defined in terms of kinds or classes of distinct nature (phenotype), ie ., without assigning any value. Such characters are generally simply inherited under the control of one or two so called major genes ( oligogenes ) and are insensitive to environments. Therefore, their genetic behaviour can be wholly accounted for on the principles of Mendelian genetics Substantial evidences on record indicate that qualitative traits are, by and large, highly heritable. Therefore, they are highly amenable to improvement by simple plant breeding methods, like selection, backcrossing or mutation, which requires no complicated programmes

12. Many traits are genetically influenced, but do not show single-gene ( Mendelian ) patterns of inheritance. They are influenced by the combined action of many genes and are characterized by continuous variation . These are called polygenic traits. Continuously variable characteristics that are both polygenic and influenced by environmental factors are called multifactorial traits. Examples of quantitative characteristics are height, weight. A quantitative trait shows continuous variation . If several gene effects are present, the phenotype values for a population will typically have a normal distribution . The genes ALSO follow Mendelian laws of inheritance; however, multifactorial traits have numerous possible phenotypic categories. BIOMETRICS IN PLANT BREEDING - QUANTITATIVE TRAIT

13. Biometry or biometrics is the science that deals with the application of statistical concepts and procedure to the study of biological problems. Biometrical genetics is that branch of genetics, which attempts to unravel the inheritance of quantitative traits using statistical concepts and procedures . It is also known as quantitative genetics. Biometrical techniques: Various statistical procedures employed in biometrical genetics are known as biometrical techniques. BIOMETRICS IN PLANT BREEDING BIOMETRICAL GENETICS

14. BIOMETRICS IN PLANT BREEDING In plant breeding progrmmes selection of the plants is based on the appearance/ phenotype. The phenotype consists of the heritable component i.e., genotype and the non-heritable component the environment. The value of phenotype would largely depend on the heritable component i.e, the genotype

15. Phenotypic variation It is the total variability, which is observable . It includes both genotypic and environmental conditions. Such variation is measured in terms of phenotypic variance. Genotypic variation It is the inherent or genetic variability, which remains unaltered by environmental conditions. This type of variability is more useful to a plant breeder for exploitation in selection or hybridization. Such variation is measured in terms of genotypic variance. The genotypic variance consists of additive, dominance and epistatic components. Environmental variation It refers to non-heritable variation , which is entirely due to environmental effects and varies under different environmental conditions. This uncontrolled variation is measured in terms of error mean variance. The variation in true breeding parental line and their F1 is non-heritable. BIOMETRICS IN PLANT BREEDING – COMPONENTS OF VARIATION

16. An estimate of the magnitude of contribution of genotype to phenotype may be obtained by using mean squares from analysis of variance Source of Variation df SS MSS EMSS Cal F Varieties G-1 GSS GSS/(g-1)  2 e+R 2 g Replication R-1 RSS RSS/(r-1)  2 e+G 2 r Error (R-1)(G-1) ESS ESS/(r-1)(g-1)  2 e Total (RG)-1 TSS ANOVA

17. 1. C.F. (Correction Factor) = GT 2 N g r 2. TSS (Total SS) = Σ Σ X 2 ij - CF i=1 j=1 r 3. RSS = Σ R 2 j / g - CF j=1 g 4. GSS = Σ T 2 i / r - CF i=1 5. ESS= TSS – RSS – GSS Sum of Squares (SS)

18. RMS = RSS/r-1 GMS = GSS/g-1 EMS = ESS/(r-1)(g-1) Calculated ‘F’ = GMS/EMS Mean Sum of Squares (MSS)

19. From expectations, we can get the following estimates of various components σ 2 (Error variance) = EMS = σ 2 e σ 2 (Genotypic variance) = (GMS – EMS) / r = σ 2 g σ 2 ( Phenotypic variance ) = σ 2 g + σ 2 e = σ 2 p Estimates of variances σ 2 p = σ 2 g + σ 2 e h 2 BS (Heritability in broad sense) = σ 2 g / σ 2 p

20. Component of Genetic Variance V G = V A + V D + V I V P = V G + V E V A = Additive component , is difference between two homozygotes . V D = Dominance component is due to the deviation of heterozygote ( Aa ) phenotype from the average of phenotypic values of two homozygotes V I = Interaction or Epistatic component , is due to interaction of two or more genes

21. Components of genetic variance Fisher was the first to divide the genetic variance into additive, dominance and epistatic components. These are briefly described below. Refers to the action of the genes affecting a trait so that each enhances the effect of the other. If in the expression of the quantitative trait, yield eg : the effect of single gene adds one increment, two genes two units and so on. aabb =0, Aabb =1, AAbb =2, AABb =3, AABB=4 etc It is the component which arises from differences between two homozygotes of a gene, i.e., AA and aa . The additive genetic variance is associated with homozygosity and, therefore, it is expected to be maximum in self-pollinating crops and minimum in cross-pollinating crops. Additive variance is fixable and, therefore, selection for traits governed by such variance is very effective. BIOMETRICS IN PLANT BREEDING – COMPONENTS OF VARIATION ADDITIVE VARIANCE

22. It is due to the deviation of heterozygote ( Aa ) from the average of two homozygotes (AA and aa ). Such genes show incomplete or over-dominance or complete dominance . With complete dominance, the heterozygote and homozygote have equal effects. eg : aa =0, Aa =2, AA=2. The dominance variance is associated with heterozygosity and, therefore, it is expected to be maximum in cross-pollinating crops and minimum in self-pollinating species . Dominance variance is not fixable and, therefore, selection for traits controlled by such variance is not effective . Heterosis breeding may be rewarding in such situation. DOMINANCE VARIANCE

23. EPISTATIC VARIANCE It arises due to the deviations as a consequence of inter-allelic interaction , i.e. , interaction between alleles of two or more different genes or loci . Two genes may have no effect individually but still have an effect when combined. eg : AAbb =0, aaBB =0, AABB=4. The epistatic variance is of three types viz., additive x additive, additive x dominance and dominance x dominance (Mather and Jinks ).

24. In crop improvement only the genetic component of variation is important since only this component is transmitted to the next generation . Heritability is the ratio of genotypic variance to the phenotypic variance . Heritability denotes the proportion of phenotypic variance that is due to genotype i.e., heritable . It is generally expressed in percent (%) It is a good index of transmission of characters from parents to their offspring

25. TYPES OF HERITABILITY Depending upon the components of variance used as numerator in the calculation ,there are 2 definitions of Heritability 1. Broad sense heritability Narrow sense heritability

26. Broad sense heritability According to Falconer, broad sense heritability is the ratio of genotypic variance to total or phenotypic variance It is calculated with the help of following formula where , V g = genotypic variance V p = phenotypic variance V e = error variance Heritability ( h² ) = V g / V p x 100 = V g / V g + V e x 100

27. Broad sense heritability B road heritability ( h 2 ) separates genotypic from environmentally induced variance : h 2 = V g / V p It can be estimated from both parental as well as segregating populations It express the extent to which the phenotype is determined by the genotype , so called degree of genetic determination It is most useful in clonal or highly selfing species in which genotypes are passed from parents to offspring more or less intact It is useful in selection of superior lines from homozygous lines

28. Narrow sense heritability In outbreeding species evolutionary rates are affected by narrow- sense heritability It is the ratio of additive or fixable genetic variance to the total or phenotypic variance Also known as degree of genetic resumblance it is calculated with the help of following formula where V A or D = additive genetic variance V P or VP = phenotypic variance Heritability ( h² ) = V A / V P x 100 or ½ D / VP

29. NARROW SENSE HERITABILITY It plays an important role in the selection process in plant breeding For estimation of narrow sense heritability , crosses have to be made in a definite fashion It is estimated from additive genetic variance It is useful for plant breeding in selection of elite types from segregating populations

30. If heritability in broad sense is high It indicates character are least influenced by environment selection for improvement of such characters may be useful If heritability in broad sense is low The character is highly influenced by environmental effects Genetic improvement through selection will be difficult

31. If heritability in narrow sense is high characters are govern by additive gene action Selection for improvement of such characters would be rewarding If low heritability in narrow sense Non additive gene action Heterosis breeding will be beneficial

32. H 2 varies from 0 (all environment) to 1 (all genetic) Heritability of are found in highly inbred populations with no genetic variation. Heritability of 1 are expected for characters with no environmental variance in an outbred population if all genetic variance is additive. Heritability are specific to particular populations living under specific environmental conditions Heritability (h²) and Additive Variance (V A ) are fundamentally measures of how well quantitative traits are transmitted from one generation to the next

33. Type of genetic material : the magnitude of heritability is largely governed by the amount of genetic variance present in a population for the character under study Sample size : Large sample is necessary for accurate estimates Sampling methods : 2 sampling methods , Random and Biased . The random sampling methods provide true estimates of genetic variance and hence of heritability

34. Layout or conduct of experiment : Increasing the plot size and no. of replications we can reduce experimental error and get reliable estimates Method of calculation : heritability is estimated by several methods Effect of linkage : high frequency of coupling phase (AB/ab) causes upward bias in estimates of additive and dominance variances Excess of repulsion phase linkage (Ab/aB ) leads to upward bias in dominance variance and downward bias in additive variances

35. It is useful in predicting the effectiveness of selection. It is also helpful for deciding breeding methods to be followed for effective selection. It gives us an idea about the response of various characters to selection pressure. I t is us e f ul in p re dic t i n g th e p e rforma n ce u n d e r dif f e rent degree of intensity of selection. It helps for construction of selection index. Estimates of heritability serve as a useful guide to the breeder, to appreciate the proportion of variation that is due to genotypic or additive effects.

36. Improvement in the mean genotypic value of selected plants over the parental population is known as genetic advance It is the measure of genetic gain under selection The success of genetic advance under selection depends upon three factors (Allard , 1960) Genetic variability : greater the amount of genetic variability in base populations higher the genetic advance Heritability : the G.A. is high with characters having high heritability Selection intensity : the proportion of individuals selected for the study is called selection intensity . high selection intensity gives better results

37. The genetic advance is calculated by the following formula where , K = standardize selection differential h² = heritability of the character under selection δ p = phenotypic standard deviation The estimates of GS have same unit as those of the mean The genetic advance from mixture of purelines or clones should be calculated using h² (bs) From segregating populations using h² (ns ) GS = K x h² x δ p

38. If the value of Genetic advance high The character is governed by additive genes and selection will be beneficial for such traits If Genetic advance is low The character is governed by non additive genes and heterosis breeding may be useful Interpretation of Genetic advance

39. The external condition that affects the expression of genes of genotype Comstock and Moll, 1963 classified in two groups Micro environment : environment of single organism , as opposed to that of another growing at the same time and place e.g. physical attributes of soil , temp , humidity , insect-pests and diseases Macro environment : associated with a general location and period of time . A collection of micro environment

40. Allard and Bradshaw ,1964 classified Environmental variables into two groups Predictable or controllable environment : includes permanent features of environment ( climate , soil type, day length) controllable variable : fertilizer level, sowing date & density, methods of harvesting . High level of interaction is desirable Unpredictable or uncontrollable environment : difference between seasons, amount & distribution of rainfall, prevailing temperature . Low level of interaction is desirable

41. Algebraically, we can define the phenotypic value Of an individual as the consequence of the alleles It inherits together with environmental influences As P = G + E P = G + E + G x E Where P = phenotype, G = Genotype, and E = Environment

42. A phenotype is the result of interplay of a genotype and each environment . A specific genotype does not exhibit the same phenotypic characteristics under all environment, or different genotype respond differently to a specified environment. This variation arising from the lack of correspondence between genetic and non genetic effects is known as Differences in performance of genotypes in different environments is referred to as . The low magnitude of genotype x environment interaction indicates consistence performance of the population .Or it shows high buffering ability of the population