36.Kovacs reagent is used in Indole test. Kovacs reagent is 4 (p)-.pdf

Since the heat of reaction ..is negative,.... it loses heat to.. the surroundings and
therefore.. is exothermic. 2 NO(g) + O2(g) ---> 2 NO2(g) change in H= -114.1kJ molecular
weight of NO2=80 so no of moles= 113.8/80=1.4225 so amount of heat produced =-
114.1/(2*1.14225) =40 kJ
Solution
Since the heat of reaction ..is negative,.... it loses heat to.. the surroundings and
therefore.. is exothermic. 2 NO(g) + O2(g) ---> 2 NO2(g) change in H= -114.1kJ molecular
weight of NO2=80 so no of moles= 113.8/80=1.4225 so amount of heat produced =-
114.1/(2*1.14225) =40 kJ.

1. 36.Kovac\'s reagent is used in Indole test. Kovacs reagent is 4 (p)-dimethylamino benzaldehyde.
Indole test is used to determine the ability of an organism to split indole ring from tryptophan.
Tryptophan is hydrolysed by tryptophanase to produce indole. Indole production is detected by
Kovac’s reagent with the formation of red color in the culture broth.
37. Oxidase. Oxidase test is done to detect the presence of cytochrome oxidase presence.
N,N,N,N-tetramethyl-p-phenylenediamine (TMPD) is the reagent used. Purple color production
indicates positive result.
38. H2O2 is used in Catalase test. This is done to detect the production of catalase by the
bacteria. Catalase cleaves H2O2 into H2O and O. The nascent oxygen produced escapes in the
form of bubbles.
39. IMViC test was done. I is for Indole, M for Methyl Red, Vi for Vogues Proskeur and C is for
Citrate. IMViC test is done to differentiate Enterobacteriaceae members.
40. The probable organism as per the reactions is Citrobacter freundii
41. Carbol fuschin is the primary stain used in acid fast staining. Sulphuric acid is used as
decoloriser and methylene blue is the secondary or counter stain.
42. Crystal violet is used for Grams stain. It is the primary stain.

Solution

36.Kovac\'s reagent is used in Indole test. Kovacs reagent is 4 (p)-dimethylamino benzaldehyde.
Indole test is used to determine the ability of an organism to split indole ring from tryptophan.
Tryptophan is hydrolysed by tryptophanase to produce indole. Indole production is detected by
Kovac’s reagent with the formation of red color in the culture broth.
37. Oxidase. Oxidase test is done to detect the presence of cytochrome oxidase presence.
N,N,N,N-tetramethyl-p-phenylenediamine (TMPD) is the reagent used. Purple color production
indicates positive result.
38. H2O2 is used in Catalase test. This is done to detect the production of catalase by the
bacteria. Catalase cleaves H2O2 into H2O and O. The nascent oxygen produced escapes in the
form of bubbles.
39. IMViC test was done. I is for Indole, M for Methyl Red, Vi for Vogues Proskeur and C is for
Citrate. IMViC test is done to differentiate Enterobacteriaceae members.
40. The probable organism as per the reactions is Citrobacter freundii
41. Carbol fuschin is the primary stain used in acid fast staining. Sulphuric acid is used as
decoloriser and methylene blue is the secondary or counter stain.
42. Crystal violet is used for Grams stain. It is the primary stain.