3d bioprinting using tissue and organ .pptx

This is ppt about 3d bioprinting of tissue and organ

1. Title: 3D Bioprinting Using Tissue and Organs Subtitle: Revolutionizing Regenerative Medicine Presented by: Naziya Khan Institute : Guru Ghasidas Vishwavidhyalaya

2. Introduction to 3D Bioprinting Definition of 3D Bioprinting Importance in regenerative medicine Applications in tissue and organ replacement Combining 3D printing, biomaterials, and cellular biology

3. Definition of 3d bioprinting 3D bioprinting is an additive manufacturing process that uses bioinks to print living cells developing structures layer-by-layer which imitate the behavior and structures of natural tissues A process that creates three-dimensional objects from a digital design, layer by layer, using materials like plastics, metals, or ceramics.  A brief history of bioprinting . In 1984, Charles Hull invented stereolithography (SLA) for printing 3D objects from digital data, symbolizing the birth of 3D printing.

4. what are bioinks ? Bioinks are essentially "inks" that contain living cells and other biomaterials, allowing for the precise 3D placement of cells or molecules within a construct.  Purpose: They are used in bioprinting , a process that utilizes 3D printing techniques to create engineered tissues and organs.  Key properties: Printability:   Bioinks need to be printable and have the right rheological properties (flow behavior) for extrusion-based bioprinting .  Biocompatibility:  They must be biocompatible, meaning they should not induce an adverse immune response or toxicity in the cells.  Mechanical properties:   Bioinks should have adequate mechanical properties to support the printed structure and mimic the tissue's natural environment. 

5. How 3D Bioprinting Works Step 1: Pre- Bioprinting (Preparation Phase) Imaging and 3D modeling Bio-ink selection (living cells, biomaterials) Step 2: Bioprinting Process Extrusion-based, inkjet, laser-assisted, and stereolithography bioprinting Step 3: Post- Bioprinting (Maturation Phase) Cell culturing and vascularization

6. Process of 3D bioprinting This figure outlines the step-by-step process of 3D bioprinting used in hepatic tissue engineering. The process includes bioink preparation, scaffold design, printing, and post-printing maturation

7. Types of 3D Bioprinting Techniques Extrusion-Based Bioprinting : Layer-by-layer cell deposition Inkjet Bioprinting : Droplet-based cell placement Laser-Assisted Bioprinting : High precision cell placement Stereolithography Bioprinting : UV-light solidification of bio-ink

8. Materials Used in 3D Bioprinting Bio-inks: Hydrogels with living cells Scaffold Materials: Gelatin, alginate, collagen Cell Sources: Stem cells, patient-derived cells Growth Factors & Nutrients: Support cell survival and function

9. Applications of 3D Bioprinting Tissue Engineering: Skin, cartilage, bone regeneration Organ Printing: Experimental heart, liver, kidney tissues Drug Testing & Development: Reduced reliance on animal testing Cancer Research: Bioprinted tumors for personalized treatment

10. A) Inkjet-based, B) extrusion-based, C) laser-assisted, D) stereolithography -based, and E) in situ inkjet bioprinting processes. F) Morphological and histological features of artificial skin fabricated using extrusion-based 3D bioprinting G) Artificial bone disk fabricated using the in situ H) Artificial vascular graft fabricated using stereolithography -based 3D bioprinting

11. Challenges and Limitations Vascularization : Difficulty in blood vessel formation Cell Viability: Ensuring survival and proper function Regulatory Issues: FDA and ethical concerns Cost and Scalability: High costs and production limitations

12. Future of 3D Bioprinting AI and machine learning integration Fully functional bioprinted organs CRISPR and gene editing applications Expansion in regenerative therapies

13. Conclusion 3D bioprinting is a game-changer in medicine Potential for patient-specific organ replacements Ongoing research is pushing the limits of possibility