Paper Chromatography Aarushi Chakraborty Class 7 E CHEMISTRY PROJECT
WHAT IS PAPER CHROMATOGRAPHY ? Paper chromatography is an analytical method used to separate coloured chemicals or substances. It is now primarily used as a teaching tool, having been replaced in the laboratory by other chromatography methods such as thin-layer chromatography. The setup has three components.
HOW IS IT CARRIED OUT ? WHAT KIND OF PAPER IS USED ? WHAT IS THE RESULTING IMAGE CALLED ? Paper chromatography is a technique used to separate mixtures into their individual components. It's often employed in chemistry labs and in fields like biochemistry and forensics. To carry out paper chromatography, a small spot of the mixture to be analysed is placed near the bottom of the chromatography paper. The paper is then suspended vertically in a container with a solvent, which acts as the mobile phase. As the solvent moves up the paper by capillary action, it carries the components of the mixture with it. Different components of the mixture travel at different rates based on their affinity for the paper and the solvent. This differential migration causes the components to separate into distinct bands on the paper In paper chromatography, a piece of special paper, known as chromatography paper, is used as the stationary phase. This paper is usually made of cellulose and has a very uniform structure to ensure consistent results. The resulting image, showing the separated components as bands or spots on the paper, is called a chromatogram.
DIFFERENT TYPES OF CHROMATOGRAMS
WHAT ARE THE DIFFERENT TYPES OF SOLVENTS THAT CAN BE USED ? In paper chromatography, the choice of solvent is crucial as it directly affects the separation of components. Different solvents have different polarities, which interact differently with the components of the mixture. Some common types of solvents used in paper chromatography include: 1. Water 2. Ethanol 3. Methanol 4. Acetone 5. Butanol 6. Acetic acid 7. Hexane 8. Chloroform Each solvent has its own set of advantages and limitations, and the selection depends on the nature of the mixture being analysed and the desired separation results. It's often necessary to experiment with different solvent systems to achieve optimal separation.
ACKNOWLEDGEMENT I would like to thank Mrs. Rupika Nath for providing me the opportunity to make a wonderful project on Paper Chromatography. This Photo by Unknown Author is licensed under CC BY-NC REFERENCE : www.google.com www.Britannica.com www.amazingscientists.com www.youtube.com www.sciencedirect.com