Aas mujtaba

AAS

1. PRESENTATION ON: PRINCIPLE OF ATOMIC ABSORPTION SPECTROSCOPY(AAS ) INTEGRAL UNIVERSITY,LUCKNOW Session: 2016-2017 Presented by: Under the guidence of: Mohd Muztaba M.Pharm Mr . Paramdeep Bagga Pharmacology

2. INTRODUCTION Alan walsh in mid 1950s One element can be estimated at time Qualitative determination of trace metal in liquid Detection of element 1 PPM No sample preparation No role of temperature

3. HISTORY: The technique was introduced in 1955 by Alan Walsh in Australia ( 1916 – 1998 ).

4. PRINCIPLE When a solutin containing metallic species is introduced into flame,the vapour of the metal atom will be obtained some of the metal atom will be raised to a energy level to sufficient high to emitt the characterstic rediation of a metal A phenomena that utilized in the familiar technique a emission flame photometry But a large percentage of metal atom will remain in the non emitting ground state these ground state atom of a particular element are receptive of light radiation of there on specific resonsnce wavelength

5. When a light of this wavelength is to pass through a flame having atom of the metallic species path of that light will be observed and the absorption will directly propotional to the density of atom in the flame Once this value of absorption is known the concentration of metallic element can be known because the absorption is directly propotional to the density of atom in the flame Mathemetically the total amount of light absorb may be given by expression

6. V is the total amount of light absorb e change on electron on mass c speed out light N total number of atom f Oscillator strength π e are constant

7. APPLICATION: Sr.no Name of the substance Element assay Measured at nm 1 Ascorbic acid Fe 248.3nm 2 Cispalstin Ag 328nm 3 Glucagon Zn 213.9nm 4 Oxy prenol hydrochloride Pb 217nm 5 Parazosin hydrochloride Ni 232nm 6 Zinc Oxide Cd Pd 228.7nm 217nm

8. Assay of total zinc in insulin suspension Assay of palledeum in carbenicillin sodium Assay of lead in petrol Assay of pharmaceutical substance by AAS

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