Active site of enzyme

Active sites of the enzyme is that point where substrate molecule bind for the chemical reaction. It is generally found on the surface of enzyme and in some enzyme it is a “Pit” like structure
The active site is a three-dimensional cleft formed by groups that come from different parts of the amino acid sequence
The active site takes up a relatively small part of the total volume of an enzyme
Active sites are clefts or crevices
Substrates are bound to enzymes by multiple weak attractions.
The specificity of binding depends on the precisely defined arrangement of atoms in an active site.

1. Active sites of enzyme by Avinash Tiwari M. Tech, biotechnology 2 nd year (3 rd sem.) 201710902010002

2. CONTENT Introduction Feature of the active site Structure Binding site Models for active site of enzyme Properties that Affect Binding Mechanism of catalytic site

3. INTRODUCTION Active sites of enzyme is that point where, substrate molecule bind for the chemical reaction It is generally found on the surface of enzyme and in some enzyme it is a “Pit” like structure Active site

4. Feature of the active site The active site is a three-dimensional cleft formed by groups that come from different parts of the amino acid sequence The active site takes up a relatively small part of the total volume of an enzyme Active sites are clefts or crevices Substrates are bound to enzymes by multiple weak attractions. The specificity of binding depends on the precisely defined arrangement of atoms in an active site .

5. STRUCTURE OF ENZYME

6. Binding site Form the 4 type of bond with substrate Electrostatic bond Hydrogen bond Van Der Waals forces Non- covalent bond

7. Models for active site of enzyme Lock and key theory Induced fit model

8. Properties that Affect Binding Complementarity Flexibility Surfaces Non-Covalent Forces Affinity

9. MECHANISM CATALYTIC SITE