Agilent 5500 handheld presentation.pptx

Agilent 5500 handheld presentation

1. Agilent 5500 ftir presentation Adista sn Service engineer

2. Apa itu radiasi sinar infra merah ? Gelombang electromagnet dengan jarak ( rentang ) dari limit terendah ke limit tertinggi (0.76 - 0.8 m m to an upper limit of 1 mm) Infrared radiation

3. ELECTROMAGNETIC SPECTRUM UV VIS NIR IR Far IR Microwave Wavelength (cm) 10 -5 10 -5 10 -4 10 -3 10 -2 10 -1 Energy 0.78 2.5 25 1000 Wavelength ( m m) Wavenumber (cm -1 ) 12,800 4000 400 10

4. Sifat partikel cahaya Planck’s Equation

5. Sifat gelombang dan partikel cahaya E = hc / l = h n E = Energy (ergs) h = Planck’s Constant (erg sec) c = Speed of light (cm/sec) l = Wavelength (cm) n = Frequency (cycles/sec)

6. Penyerapan dan radiasi sinar infrared Ketika suatu molekul disinari dengan radiasi elektromagnetik (infrared), maka ikatan molekul yang bergetar akan menyerap energi jika frekuensi cahaya sesuai dengan frekuensi ikatan yang bergetar ( frekuensi getarannya ).

7. Cuvette containing analyte Incident light I Transmitted light I 1 Optical path length From light source To detector Transmittance T = I I 1 INFRARED ABSORPTION dan hukum BEER’S

8. Moving Mirror Fixed Mirror Reference Laser IR Source Laser Detector Sample Cell IR Detector FTIR – how does it work?

9. FT-IR Running a sample 9 Run Sample Collect Background Check Signal

10. Components – IR Sources Source element (Front view) (Side view) Wavenumbers (cm -1 ) 50,000 40,000 30,000 20,000 10,000 5,000 * Ceramic 9,800 – 50 Tungsten-Halogen 25,000 – 2,100 *Silicon Carbide - MIR 10

11. Components – Beamsplitters Wavenumbers (cm -1 ) 50,000 40,000 30,000 20,000 10,000 5,000 Near-IR Quartz 25,000 – 2,750 UV Quartz 50,000 – 4,000 Potassium Bromide 7,500 – 400 ♠ Calcium Fluoride 10,000 – 1,700 ♠ Cesium Iodide 4,000 – 225 6.25 μm Mylar 60 – 10 12.5 μm Mylar 5 – 5 25 μm Mylar 600 – 30 50 μm Mylar 360 – 20 125 μm Mylar 360 – 10 Metal Mesh 600 – 25 ♠ denotes bridging beamsplitter 11

12. FT-IR Beamsplitter Permukaan Aktif Beamsplitter , di dukung pelat dan pelapis yang dioptimalkan untuk berbagai aplikasi dan daerah . Bagian permukaan mungkin memiliki kombinasi lapisan yang berbeda untuk laser HeNe . Mid-infrared: Ge/KBr, Ge/BaF 2 , ZnSe . Near-infrared: FeO / Quartz, (Ge/ KBr will cover part of NIR) Far-infrared: Mylar films, Metal mesh, Si. Cleaning a beamsplitter : DON’T! 12 Typical FT-IR Beamsplitter KBr substrate Ge Ge

13. Components – Detectors Wavenumbers (cm -1 ) 50,000 40,000 30,000 20,000 10,000 5,000 Silicon 25,000 – 8,600 Wavenumbers (cm -1 ) 50,000 40,000 30,000 20,000 10,000 5,000 Silicon 25,000 – 8,600 Lead Selenide ( PbSe ) 11,000 – 2,000 Wavenumbers (cm -1 ) 12,500 10,000 7,500 5,000 2,500 1,250 Narrow Band MCT ( HgCdTe ) 12,000 – 700 Broad Band MCT 11,000 – 450 DLaTGS 11,000 –185 Mid-IR Detectors Far-IR Detectors Far-IR DLaTGS 600 – 20 UV-VIS Detectors Near-IR Detectors Mid-IR & Far-IR Detectors ( Deuterated L- Alanine Tellurium Gallium Sulphide ) 13

14. Product Portfolio – In Lab FTIR Analyzers Physical Attributes 8 lbs 8” x 8” x 4.5” External Computer USB connection External Power Full spectral analysis

15. Analisa Sampel Padat dengan FTIR Metoda Dialpath Metoda Diffuse reflectance Metoda attenuated total reflectance tipe Horizontal

16. BAGAIMANA ANALISA DENGAN FTIR? Kebutuhan pretreatmen sampel bergantung pada masing-masing analisa . Atur parameter pengukuran . Lakukan koreksi background menggunakan reference material atau udara . Lakukan pengukuran sampel .

17. Analisa dengan atr (attenuated total reflectance) Pengukuran - Sampel diletakkan hingga kontak dengan prisma yang terbuat dari materi ber -RI tinggi yang dapat mentransmisikan sinar IR. - Sudut datang IR beam lebih besar dari sudut kritis sehingga IR beam direfleksikan secara total diantara sampel dan prisma .

18. Diamond ATR

19. What is ATR? Attenuated Total Reflectance ATR crystal IR Energy penetrates the surface of the sample sample

20. Kedalaman penetrasi atr prisma sample sinar IR prisma sample dp

21. Diamond ATR For solids , liquids , powders or gels most common sample interface no sample preparation Single reflection, nominal angle = 45° , Diamond Slip clutch press provides consistent pressure Spectral range: 5,100–600 cm-1 (Cary 5500 ZnSe) Spectral range: 7,000–350 cm-1 (Cary 5500 KBr)

22. TumblIR ( single pathlength transmission )

23. TumblIR Single Transmission Cell Fixed path length liquid transmission cell Standard 100µm Can be special ordered 50µm - 200µm Liquids only Quantitative analysis 50 ppm to 5 % Reproducible and easy to use 5500t FTIR

24. TumblIR ( single pathlength transmission ) Especially suited to oil and fuel analysis Fixed 100 μ m pathlength • Quantitative analysis LOD ~50 ppm (sample dependent) • Transmission measurement • Liquids and thin polymer films (< 50 μm thick) • Fixed pathlength = 100 μm factory calibrated • Repeatability = ± 0.25 μ m • ZnSe windows • Spectral range : 5,100–600 cm-1

25. Single Transmission Cell Operation 1. Place Sample on Lower Window 3. Once in place analyze the sample 2. Rotate into place 4. Cleaning is easy!

26. DialPath ( multipath liquid transmission )

27. DialPath ( multipath liquid transmission ) Quantitative analysis LOD ~25 ppm (sample dependant with 250 μm pathlength cell) Qualitative analysis , library search Transmission measurement • Liquids and thin polymer films (< 50 μm thick) • Fixed pathlengths —set of three factory-calibrated 30, 50, 100 μ m or 50, 100, 250 μ m or custom pathlengths • Repeatability = ± 0.25 μ m • ZnSe windows • Spectral range : 5,100–600 cm-1

28. DialPath Operation Agilent Confidential Pathlength ( μ m ) Typical Conc. Range 30 Neat -  0.1% 50 50% - 500 ppm 100 20% - 100 ppm 200 10 % - 50 ppm

29. Perawatan dasar PERAWATAN SPEKTROSKOPI FOURIER TRANSFORM INFRARED ( ftir ) Pertahankan temperatur dan kelembaban ruangan Tingkat kelembaban : 65% or below temperatur : 15 to 30 o C Periksa kondisi silica gel secara teratur Lakukan performance test dan cek diagnostics Lakukan validasi instrument

30. Mingguan ( penggantian silica gel) Silica gel berfungsi untuk mengabsorbsi kelembaban dalam interferometer Kondensasi didalam kompartemen dapat merusak interferometer. Gunakan silica gel dengan indikator Silica gel dapat digunakan kembali dengan pemanasan dalam oven pada suhu ~50 to 80 o C.

31. troubleshooting Power spectrum rendah atau nol ( kemungkinan penyebab ) Kerusakan light source kabel light source putus ( menunjukkan berakhirnya massa pemakaian ) Interferometer mis-alignment menyebabkan deviasi kondisi interferensi optimum Gangguan pada interferometer Gangguan pada unit detector temperature control kerusakkan laser source Gangguan lain pada komponen sirkuit .

32. solutions lakukan self test/diagnostics pada software untuk melihat apakah rendahnya nilai power disebabkan oleh interferometer mis-alignment. Ganti light source Disebabkan karena kerusakan light source atau berakhirnya masa pemakaian light source. ( agilent factory site) Perbaikan Disebabkan karena mis-alignment atau kerusakan bagian lain yang membutuhkan perbaikan ( agilent factory site)

33. Thank you