amino Acid production.pptx

Amino Acid production

1. Welcome To Our Presentation. Presentation Topic Amino Acid Production Prepared by: Group-5 Md Rayhan ID-192046031 Abu Riad ID:192047032 Md Rakibul Hasan ID-192050031 Atiq Asif Shaown ID-192052031 Amirun Akter Zahan ID-192053031

2. What is Amino acid? Amino acids are the building blocks of proteins. They are the group of organic compounds containing two functional groups- amino and carboxyl. The amino group (-NH2) is basic while the carboxyl group (-COOH) is acidic in nature. And a side-chain (R group) specific to each amino acid is also present.

3. Classification: All the 20 amino acids are not needed to be taken in the diet. Based on the nutritional requirements amino acids can be classified into two classes: - Essential and - Nonessential

4. Essential amino acids The amino acids which cannot be synthesized by the body and need to be supplied through the diet are called essential amino acids. The ten amino acids listed below are essential for humans: Arginine , Valine , Histidine , lsoleucine , Leucine , Lysine, Methionine , Phenylalanine, Threonine , Tryptophan.

5. Non essential amino acids They are synthesized by the body, and do not need not be supplied in the diet. These are- glycine , alanine , serine, cysteine , aspartate , asparagnie , glutamate, glutamine, tyrosine and proline . Some microorganisms are capable of producing certain amino acids such as lysine, glutamic acid and tryptophan.

6. Industrial Production of Amino Acids

7. Figure: Large number of microorganisms were isolated from nature which are capable of producing amino acids by fermentation in commercially feasible quantities, especially from the auxotrophic bacteria

8. The details of production of different amino acids by fermentation involving different microorganisms are precised in this Table..

9. Industrial Production of Amino Acids

10. Industrial Production of Amino Acids Two amino acids which are produced at large scale includes- L-lysine L- glutamic acid

11. Lysine Production lysine is the amino acid that covers more than 90% of total world amino acid production. Synthesis of lysine – 80% by Fermentation. 20% by chemical synthesis. Fermentation Process - Submerged Fermentation Aerobic Fermentation Mode of Operation - Batch Process Fed-Batch Process . Fermenter Type - Stirred Tank Reactors Air Lift Bioreactors

12. Lysine Production L-lysine, 2, 6-diaminohexanoic acid, is synthesized by microorganisms either via diaminopimelic acid pathway or the aminoadipic acid pathway. However, in any single organism only one of the two alternative pathways is employed. Bacteria, actinomycetes , cyanobacteria (Blue-green algae), some phycomycetes and protozoa use the DAP ( Diaminopimelic acid) pathway while some phycomycetes , all ascomycetes and basidiomycetes and eukaryotic algae uses the aminoadipic acid pathway.

13. E.Coli Auxotroph

14. L-lysine

15. L- glutamic acid production Corynebacterium glutamicum , is a short, aerobic, Gram-positive rod capable of growing on a simple mineral salt medium with glucose, provided that biotin is also added. Production of L- glutamic acid by C. glutamicum is maximal at a critical biotin concentration of 0.5 mg g -1 of dry cells, which is suboptimal for growth Detergents like Tween-40, addition of penicillin, use of Glucose, Glucose-6P, CO2, fatty acid auxotrophic strains, or addition of ethambutol - inhibiting arabinogalactan synthesis.

16. L- Glutamin Acid production Flow Chart

17. Thank You All…