animal like protist the protozoa from animal form and fuction

presentation on the topic of the animal like protist the protozoa

1. The protozoa

2. 1. Fossil study of protozoans: The fossil record indicates that all protists and animal phyla were present during the Cambrian period, about 550 million years ago. Unfortunately, only a few fossil evidence are available. Therefore, scientists gather evidence by examining the structure and function of living species. 2. Origin of protists: Ancient Archaea were the first living organisms on this planet. The Archaea gave rise to the kingdom Protista about 1.5 billion years ago. The endosymbiont hypothesis explains the mechanism of this evolution of protista. Most scientists agree that the protists probably arose from more than one ancestral Archaean group.

3.  3. Taxonomy of protozoans : Zoologists recognize between 7 and 45 phyla of protists. These phyla represent numerous evolutionary lineages. Therefore, the protists are polyphyletic. Kingdom protista has two groups Pla n t li k e p r o ti s ta ( Alg a e ): T h ese are p r i m arily autotrophic. Ani m al li k e p r otista ( p r o t o z o a ): These are primarily heterotrophic.

4. Protozoa (Gr. proto, first zoa, animal) have unicellular organization, which does not necessarily imply that they are simple organisms. In some protozoan phyla, individuals group to form colonies, associations of individuals that are not dependent on one another for most functions. Maintaining homeostasis in protozoans: Protozoans maintain homeostasis by following methods: Support Osmoregulation Ingestion of food Process of digestion Respiration and excretion Reproduction

5. The body of protozoans are supported by pellicle and cytoplasm . 1. A regular arrangement of microtubules is called the pellicle . Pellicle underlies the plasma membrane of many protozoa. The pellicle is rigid enough to maintain the shape of the protozoan, but it is also flexible. 2. The cytoplasm of a protozoan is differentiated into two regions. The portion of the cytoplasm just beneath the pellicle is called ectoplasm It is relatively clear and firm. The inner cytoplasm, called endoplasm , is usually granular and more fluid. 2. Osmoregulation Most marine protozoa have solute concentrations similar to that of their environments. However, Freshwater protozoa must regulate the water and solute concentrations of their cytoplasm. Water enters freshwater protozoa by osmosis because of higher solute concentrations in the protozoan than in the environment. Contractile vacuoles remove this excess water. These contractile vacuoles are composed of collecting tubule.

6. There are different methods of ingestion of food: Most prot o zoa abs o rb dis sol v ed nutrie n ts by acti v e transport . Some protozoa ingest whole or particle of food endocytosis . It forms food vacoules. Some protozoa ingest food in a specialized region analogous to a mouth, called the cytopharynx or gullet . 4. Process of digestion Digestion and transport of food occurs in food vacuoles that form during endocytosis . Food vacuoles fuse with enzyme-containing lysosomes and circulate through the cytoplasm, distributing the products of digestion. After digestion is complete, the vacuoles are called egestion vacuoles . They release their contents by exocytosis , sometimes at a specialized region of the plasma membrane or pellicle called the cytopyge .

7.  Protozoan have a large surface area in proportion to their volume. Both gas exchange and excretion occur by diffusion across the plasma membrane. Gas exchange involves absorption of oxygen and elimination of the carbon dioxide. Excretion is the elimination of the nitrogenous by-products of protein metabolism. By-product in protozoa is mostly ammonia . Reproduction Both asexual and sexual reproduction occurs among the protozoa. Asexual reproduction Binary fission ( mitosis produces two nuclei ) Budding ( mitosis produces a small cytoplasm along with nuclei ) Multiple fission or schizogony ( parent cell divides into multiple no. of small cells ) Sexual reproduction ( gamete formation>zygote> meiosis>mitosis ) Concern from the book. See page 106-107 of miller and harley.

8. Contents under study 2. Protozoan taxonomy

9. b i os, Many protozoa have symbiotic lifestyles. Symbiosis (Gr. syn, with life) is an intimate association between two organisms. There are following types of symbiosis in protozoans: Parasitism: An association in which one organism lives in or on a second organism, called a host, and cause disease in the host is called parasitism. Some parasites have multiple hosts. These hosts may be: Definitive host (sexual stages occur) Intermediate host (offsprings are enter into another host, and reproduce asexually in it and passes into definitive host) Commensalism: The symbiotic relationship in which one member of the relationship benefits, and the second member is neither benefited nor harmed. Mutualism: The symbiotic relationship in which both species benefit.

10. Zoologists who specialize in the study of protozoa are called protozoologists. Most protozoologists now regard the protozoa as a subkingdom, consisting of seven separate phyla within the kingdom Protista. These are the phylum Sarcomastigophora , consisting of flagellates and amoebae with a single type of nucleus. The phyla Labyrinthomorpha, Apicomplexa, Microspora, Acetospora, and Myxozoa , consisting of either saprozoic or parasitic species. And the phylum Ciliophora , containing ciliated protozoa with two types of nuclei. The classification of this subkingdom into phyla is based primarily on types of nuclei, mode of reproduction, and mechanism of locomotion Overall, the number of protozoan species exceeds 38,000.

11. Kingdom protista Subkingdom protozoa: ( single celled eukaryotes, lacking collagen and cell wall) 1. Phylum Sarcomastigophora ( About 18,000 species ) Subphylum Mastigophora Class Phytomastigophorea (Euglena, Volvox, Chlamydomonas) Class Zoomastigophorea (Trypanosoma, Leishmania, etc.) Subphylum Sarcodina ( About 4,000+3000 species ) Superclass Rhizopoda ( Amoeba, Entamoeba, Arcella ) Superclass Actinopoda (M arine radiolarians, Freshwater heliozoans) Subphylum Opalinata ( Opalina, Zelleriella) 2. Phylum Labyrinthomorpha ( Labyrinthula.) 3. Phylum Apicomplexa (Coccidians, Plasmodium) About 5,500 species. 4. Phylum Microspora ( Nosema). About 850 species. 5. Phylum Acetospora (Paramyxa, Haplosporidium). 6. Phylum Myxozoa ( The Myxozoans) About 1,250 species. 7. Phylum Ciliophora (Paramecium etc.) About 9,000 species.

12.  CLASS PHYTOMASTIGOPHOREA Dinoflagellates Flagella – 2 (1 wrap around the body & other is trailing flagellum) Pigment ( Xanthophylls) Toxins ( Red tide – Bloom ) Euglena Volvox

13. Members o f the clas s Zoom a s t ig o p h orea lack chloroplasts . They are heterotrophic. Some members of this class are important parasites of humans. One of the most important species of zoomastigophoreans is Trypanosoma brucei. This species is divided into three subspecies: T. b. Brucei (Parasite of nonhuman mammals of Africa. T. b. Gambiense (Cause sleeping sickness in humans). T. b. Rhodesiense (Cause sleeping sickness in humans). T s e t s e f l i e s ( Glos s i n a spp . ) a r e int e r m edi a te ho s ts a n d vectors of all three subspecies. Disease cycle.

14. Members of the subphylum Sarcodina (sarko-dinah) are the amoebae. When feeding and moving, they form temporary cell extensions called Pseudopodia (Gr. Pseudes - false + Podion - little foot). Pseudopodia exist in a variety of forms. Lobopodia – (mean little foot) Filopodia – (means thread) Reticulopodia – ( means netlike) Axopodia – (means axle)

15.  1. Lobopodia: They are broad cell processes containing ectoplasm and endoplasm. They are used for locomotion and engulfing food.  2. Filo p odi a : They co n t a in e c to p lasm onl y . T hey provide a constant two-way streaming movement, that delivers food in a conveyor-belt fashion. 3. Reticulopodia: They are similar to filopodia, except that they branch and rejoin to form a netlike series of cell extensions. 4. Axopodia: They are thin, filamentous and supported by a central axis of microtubules. The cytoplasm covering the central axis is adhesive and movable. Food caught on axopodia can be delivered to the central cytoplasm of the amoeba.

16. Subphylum Sarcodina>Superclass Rhizopoda>Class Lobosea. The m ost fa m i l i a r a m oe b ae b e l o ng t o t he s uper c l a s s Rhiz o pod a , c l a s s Lobosea, and the genus Amoeba. These amoebae are naked. They have no test or shell. They a re no r m a l ly found o n s hal l ow- w a t er s ubs t rates o f f r e shw a t e r ponds, lakes, and slow-moving streams. All amoeba are particle feeders, a few amoeba are pathogenic they feed on other protists and bacteria. They engulf food by phagocytosis. The process phagocytosis involves the cytoplasmic changes and food vacuoles are formed. Asexual reproduction takes place by Binary fission. Sexual reproduction is not present in them. Shelled Rhizopodans : Other members possess a test or shell. The shell may be: Made up of Calcium carbonate – Calcareous, Protein – Proteinaceous, Silica – Siliceous, Polysaccharides – Chitinous or it may be form from debris .

17.  Note: Tests are protective structures that the cytoplasm secret e s.

18.  Arcella i s a common freshwate r , shelled amoeba. It has a brown, proteinaceous test that is flattened on one side and domed on the other. Pseudopodia project from an opening on the flattened side.  Difflugia i s another common freshwater, shelled amoeba. Its test is vase shaped and is composed of mineral particles embedded in a secreted matrix.

19. Difflugia

20. A r cella

21. It causes dysentery in humans. It causes Inflammation and ulceration of the lower intestinal tract and a debilitating diarrhea – It is a blood and mucus characterize dysentery. Amoebic dysentery is a worldwide problem. It is caused due to unsanitary conditions. A significant problem in the control of Entamoeba histolytica is that an individual can be infected without experiencing symptoms of the disease. Amoebae live in the folds of the intestinal wall, feeding on starch and mucoid secretions. They pass from one host to another in the form of cysts transmitted by fecal contamination of food or water. After ingestion by a new host, amoebae leave their cysts and take up residence in the host’s intestinal wall.

22. Entamoeba histolytica

23. Foraminiferans (commonly called forams) are primarily a marine group of amoebae. Foraminiferans possess reticulopodia and secrete a test that is primarily calcium carbonate. The Foraminiferans grow and secrete new, larger chambers that remain attached to the older chambers (figure 8.13). Test enlargement follows a symmetrical pattern. It may form two types of chambers: Straight chain of chambers Spiral arrangement that resembles a snail shell. Many of these tests become relatively large; for example, “Mermaid’s pennies,”. It is several centimeters in diameter. It is found in Australia.

24. Figure 8.13

25. Their tests are abundant in the fossil record of Cambrian period. They make up a large component of marine sediments. Their tests accumulation on the floor of oceans resulted in limestone and chalk deposits. The white cliffs of Dover in England are one example of a foraminiferan-chalk deposit. Oil geologists use fossilized forams to identify geologic strata during oil exploration.

26. Heliozoans are aquatic amoebae. They are either planktonic or live attached by a stalk to some substrate . The plankton of a body of water consists of those organisms that float freely in the water i.e ., phytoplanktons & zooplankton. Heliozoans are either naked or enclosed within a test. The tests contains openings for axopodia (figure 8.14a).

27. Figure 8.14a Heliozoans

28. They are marine and freshwater amoebae. They are planktons. They are relatively large; some colonial forms may reach several centimeters in diameter. They possess a test (usually siliceous). It is composed of long, movable spines and needles. Sometimes, it forms highly sculptured and ornamented lattice (figure 8.14b). When radiolarians die, their tests settle down in the ocean floor. Some of the oldest known fossils of eukaryotic organisms are radiolarians.

29. Radiolarians Figure 8.14b

30. The very small phylum Labyrinthomorpha. They consists of protozoa with spindle-shaped, nonamoeboid, vegetative cells. In some genera, amoeboid cells use a typical gliding motion to move within a network of mucous tracks. Most of t h e m e m ber s are m arin e , and e ith e r sa pro z o ic or parasitic on algae or seagrass. Several years ago, Labyrinthula killed most of the “eel grass” on the Atlantic coast. Many ducks that feed on the grass face the starvation.

31. M e m b ers o f t h e p h ylum Apicom p lexa are all parasites. Characteristics of the phylum include: They have apical complex for penetrating host cells. They have single type of nucleus. They have no cilia and flagella, except in certain reproductive stages. The life cycles that typically include asexual stages; schizogony, sporogony and sexual stages; gametogony.

32. The most important species in the phylum Apicomplexa are members of the class Sporozoea. The class name derives from most sporozoeans producing a resistant spore or oocyst following sexual reproduction. Some members of this class, including Plasmodium and coccidians, cause a variety of diseases in domestic animals and humans. Life cycle : Many coccidian are intracellular parasites, and their life cycles have three phases. Schizogony or Merogony – Mesozoites formed by asexually (multiple fission) . They leve the host cell and start effecting other cells Gametogony – Merozoites undergo gametogony (the sexual phase), Microgametocytes + Macrogametocytes = Zygote; enclosed by oocyst . Sporogony – Zygote undergoes meiosis than mitosis.

33.  1. Malaria : Plasmodium causes malaria. It was present back in 1550 A.D. Malaria contributed significantly to the failure of the Crusades during the medieval era, and along with typhus. These diseases destroy the armies. Now over 100 million humans are annually infected by malaria. Life cycle of plasmodium: The Plasmodium life cycle involves vertebrate and mosquito hosts (figure 8.15). Schizogony occurs first in liver cells and later in red blood cells. Gametogony also occurs in red blood cells. A m o squito tak e s i n g a m eto c y t es dur i ng a m eal of blood, and the gametocytes fuse and form zygote. The zyg o te pe n e tr a t e s t h e g u t of the m osquito and transforms into an oocyst. Sporogony forms haploid sporozoites that may enter a new host when the mosquito bites the host.

34.  S y m p t o ms: The symptoms of malaria recur periodically and are called paroxysms. Chills and fever correlate with the maturation of parasites, the rupture of red blood cells, and the release of toxic metabolites. Species of Plasmodium : P. Vivax : Its paroxysms recur every 48 hours. This species occurs in temperate regions and has been nearly eradicated in many parts of the world. P. Falciparum : It causes the most virulent form of malaria in humans. Its Paroxysms are irregular. It is present in mainly tropical and subtropical regions. It remains one of the greatest killers of humanity, especially in Africa. P. Malariae: It is worldwide in distribution and causes malaria with paroxysms that recur every 72 hours. P. Ovale: It is the rarest of the four human malarial species and is primarily tropical in distribution.

35. 2) Coccidiosis : Coccidians cause coccidiosis. It is a disease of poultry, sheep, cattle, and rabbits. Two genera, Isospora and Eimeria , are particularly important parasites of poultry. 3 ) Cryp t o s p o ridium : It c a uses ch r o n ic d i ar r h ea in AIDS pat i e n t . It resi s t chlorina t i o n. It i s m o st vi r u lent in immunosuppressed individuals. 4) Toxoplasmosis : Toxoplasmosis is a disease of mammals, including humans, and birds. Sexual reproduction of Toxoplasma occurs primarily in cats. Infections occur when oocysts are ingested with food contaminated by cat feces, or when meat containing encysted merozoites is eaten raw or poorly cooked. Most infections in humans are with out any symptoms and once infection occurs than an effective immunity develops.

36. 4) Toxoplasmosis : If a woman is infected near the time of pregnancy, or during pregnancy, congenital toxoplasmosis may develop in a fetus. Congenital toxoplasmosis is a major cause of stillbirths and spontaneous abortions. Fetuses that survive frequently show signs of mental retardation and epileptic seizures. Precaution or cure measures: Congenital toxoplasmosis has no cure. Toxoplasmosis also ranks high among the opportunistic diseases afflicting AIDS patients. Steps to avoid infections by Toxoplasma include keeping stray and pet cats away from children’s sandboxes; using sandbox covers. We should not eat raw mutton or beef.

37. Phylum MYXOZOA

38.  The parasites of insects.  Me m bers o f t he phylum Micros p ora commonly called microsporidia. They are sma l l, obli g atory i ntracellu l ar parasites. I n cl u ded i n t his phy l um are sev e ral s pe c i es that parasitize beneficial insects. N o s e ma bombicus p a rasitizes silkworms (figure 8.16), causing the disease pebrine, and N. apis causes serious dysentery (foul brood) in honeybees.

39.  The movement is also shown as well.

40.  These p arasit e s have a possible role as biological control agents for insect pests. For example, the Pr otection Agen c y U.S. Environ m ental has approved and registered N. locustae for use in residual control of rangeland grasshoppers.  Recently, four microsporidian genera have been implicated in secondary infections of immunosuppressed and AIDS patients.

41. Acetospora is a relatively small phylum that consists exclusively of obligatory extracellular parasites characterized by spores lacking polar caps or polar filaments. The acetosporeans (e.g., Haplosporidium) primarily are parasitic in the cells, tissues, and body cavities of molluscs.

42. The phylum Myxozoa are commonly called myxosporeans. They are all obligatory extracellular parasites in freshwater and marine fish. They have a resistant spore with one to six coiled polar filaments. The most economically important myxosporean is Myxosoma cerebralis , which infects the nervous system and auditory organs of trout and salmon, causing whirling or tumbling disease.

43. Myxosoma cerebralis

44. fish infected by Myxosoma cerebralis

45. The phylum Ciliophora includes some of the most complex protozoa (see table 8.1). Characteristics of the phylum Ciliophora include: Cil i a t es ar e wi d ely d is tr i bu t ed in f reshw a t e r and m ar i n e environments. A few ciliates are symbiotic. They have cilia for locomotion. Cilia are also used for the generation of feeding currents in water. They have rigid pellicle. It keeps their shapes has fixed. They have distinct cytostome (mouth) structure. They h a v e d i m o rp h ic n u c l ei, typ i cal l y a l a r g e r macronucleus and one or more smaller micronuclei.

46.  Cilia: Cilia are generally similar to flagella, except that they are much shorter, more numerous, and widely distributed over the surface of the protozoan. Ciliary movements are coordinated so that ciliary waves pass over the surface of the ciliate. Many ciliates can reverse the direction of ciliary beating and the direction of cell movement. Some ciliates have evolved specialized cilia. Cilia may cover the outer surface of the protozoan. They may join to form cirri , which are used in movement. Alternatively, cilia may be lost from large regions of a ciliate.

47. Cilia and other Pellicular structures Basal bodies (kinetosomes) : It is the root of the cilia. Cilia arise from the basal bodies. Basal bodies of the adjacent cilia are interconnected. They form an e labor a te network of fiber s . These fibers anchor the cilia and give shape to the organism. Trichocysts : T richocy s ts are p e ll ic u lar st r u c tures pri m ari l y used f or protection. They are rodlike or oval organelles oriented perpendicular to the plasma membrane. In Paramecium, they have a “golf tee” appearance. The pellicle can discharge trichocysts, which then remain connected to the body by a sticky thread (figure 8.18).

48.  Mechanism of feeding: Paramecium: Some ciliates, such as Paramecium, have a ciliated oral groove along one side of the body. Cilia of the oral groove sweep small food particles toward the cytopharynx, where a food vacuole forms. When the food vacuole reaches an upper size limit, it breaks free and circulates through the endoplasm and digestion takes place. Didi n iu m : S o m e f r e e - li v ing ci l iates prey upon other protists or small animals. Prey capture is usually a case of fortuitous contact. The ciliate Didinium feeds principally on Paramecium , a prey that is bigger than itself. Didinium forms a temporary opening that can greatly enlarge to consume its prey.

49.  S u ct o rai n s : Suc t or i ans are c i l ia t es t h at li v e at t ac h ed to their substrate. They possess tentacles whose secretions paralyze prey, often ciliates or amoebae. The tentacles digest an opening in the pellicle of the prey, and prey cytoplasm is drawn into the suctorian through tiny channels in the tentacle. The mechanism for this probably involves tentacular microtubules.

50.  Genome : Ciliates have two kinds of nuclei. A large, polyploid macronucleus regulates daily metabolic activities. One or more smaller micronuclei are the genetic reserve of the cell. Asexual reproduction: Ciliates reproduce asexually by transverse binary fission and, occasionally, by budding . Budding occurs in suctorians and results in the formation of ciliated, free-swimming organisms that attach to the substrate and take the form of the adult.

51.  Sexual reproduction: conjugation Ciliates reproduce sexually by conjugation . The partners involved are called conjugants . Many species of ciliates have numerous mating types, not all of which are mutually compatible. The steps are as follows: Initial contact between individuals is apparently random, and sticky secretions of the pellicle facilitate adhesion. Ciliate plasma membranes then fuse and remain that way for several hours. The macronucleus does not participate in the genetic exchange. The macronucleus breaks up and reforms from micronuclei of the daughter ciliates.

52. The meiosis takes place in the conjugants and four haploid pronuclei are formed. The three pronuclei in each conjugate degenerate. The remaining pronuclei divides by mitosis. Thus each conjugant has now two pronuclei. The conjugants exchange pronuclei mutually. The pronuclei fuse with each other in each conjugant. The conjugants separate from each other and they are now called exconjugants. Each exconjugants undergo a series of nuclear divisions. Thus eight daughter nuclei are formed in each exconjugants. Two are degenerated. Four of the remaining six nuclei become macronuclei. Each exconjugants divides at this stage. Now each daughter nuclei has two macronuclei and one micronucleus. Each micronucleus again divides. Cytoplasmic division occurs. Thus four paramecia are formed from each exconjugant.

53. Most ciliates are free living; however, some are commensalistic or mutualistic , and a few are parasitic . Balantidium coli is an important parasitic ciliate that lives in the large intestines of humans, pigs, and other mammals. At times, it is a ciliary feeder ; at other times, it produces proteolytic enzymes that digest host epithelium, causing a flask- shaped ulcer . (Its pathology resembles that of Entamoeba histolytica.) B. coli is passed from one host to another in cysts that form as feces begin to dehydrate in the large intestine. Fecal contamination of food or water is the most common form of transmission. It is most common in the Philippines. Large numbers of different species of ciliates also inhabit the rumen of many ungulates (hoofed animals). These ciliates contribute to the digestive processes of their hosts.

54. 1.5 billion years ago 30000 species are known Protozoa with test left fossils RNA sequencing