antimicrobial activity

ppt

1. A STUDY OF ANTIMICROBIAL EFFECT OF CAROTENE AND ANTHOCYANIN FROM Daucus carota and Brassica oleracea By R. Laann swick, III B. Sc., Biotechnology *S. V. Bakiya lakshmi M. Sc., (PhD), Asst. Professor, Department of Botany & Biotechnology, Bon Secours College for women, Thanjavur.

2. WHAT IS PIGMENT? Pigments produce the colors that we observe at each step of our lives , because pigments are present in each one of the organisms in the world , and plants are the principal producers. They are in leaves , fruits , vegetables , and flowers; also present in skin , eyes , and other animal structures: and in bacteria and fungi.

3. TYPES OF PIGMENTS: NATURAL PIGMENTS SYNTHETIC PIGMENTS (natural and synthetic pigments are used in medicines , Foods , clothes , furniture , cosmetics , and in other products )

4. Natural pigments have more important functions other than the synthetic pigments. Historically ,at the beginning of the food industry consumers did not take care about the kind of pigments used in food coloring , but the “synthetic pigments” produce various side effects where the natural pigments attributed pharmacological benefits .

5. NATURAL PIGMENTS Natural pigments are classified by their structural characteristics such as e.g., Chlorophylls Carotenoids Purines Flavins Anthocyanin

6. CAROTENOIDS Carotenoids are widely known as provitamin A . Increasing interest in their role as antioxidants . ( Antioxidants – to prevent the cell damage caused by oxidants ) Anti cancer activity . The protection against cardiovascular disease . In recent studies , protective effects of carotenoid on bladder cancer .

7. ANTHOCYANIN: Anthocyanin pigments are responsible for red , purple, and blue colors of many fruits , vegetables , cereal grains , and flowers . It’s present in some foods and beverages. To play an important role in the prevention of cancer , cardiovascular disease , induction of apoptosis . ( Apoptosis – programmed cell death )

8. AIM &OBJECTIVES: To study the antimicrobial activity of carotene and anthocyanin compounds isolated from Daucus carota , Brassica oleracea . To isolate the bioactive compounds carotene and anthocyanin by thin layer chromotography .

9. MATERIALS & METHODS:

10. COLLECTION OF PLANT MATERIAL: The fresh samples of Daucus carota and Brassica oleracea were collected the 10g of plant materials were crushed and extracted the extract was filtered using Whatmann filter paper

11. ISOLATION: The extracted compounds were isolated by TLC TLC: THIN LAYER CHROMATOGRAPHY is one of the simplest techniques used to separate organic compounds and to test the purity of compounds.

12. ESTIMATION: The carotene and anthocyanin was estimated by spectrophotometer method. ( In spectrophotometer the number of particles FOOD COLORING present in a given volume of the sample increases , the light absorbance increases too)

13. ESTIMATION OF CAROTENE AND ANTHOCYANIN: S.no Sample Carotene ( % ) Anthocyanin (%) 1 Daucus carota L 25.4 2.78 2 Brassica oleracea 11.88 50.4 The maximum amount of carotene as present in Daucus carota L and the high Anthocyanin compare with Daucus carota L .

14. ANALYSIS OF ANTIMICROBIAL ACTIVITY OF Daucus carota L and Brassica oleracea phytocompounds The antimicrobial activity was assessed by disc diffusion method. The zone of inhibition was assessed and the plate were kept at room temperature for 24 hours. The isolated compound Carotene and Anthocyanin was assessed , against bacteria and fungus species.

15. ANTIBACTERIAL ACTIVITY OF CAROTENE &ANTHOCYANIN:

16. ANTIFUNGAL ACTIVITY OF CAROTENE & ANTHOCYANIN:

17. FUTURE ASPECTS:

18. CONCLUSION: This study demonstrated that phytocompounds from Daucus carota L and Brassica oleracea had significant degrees of antimicrobial activity both bacteria and fungi. Hence this preliminary investigation will be used for isolation of phytocompounds which can be used as food preservatives and pharmaceutical product development.

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