Bacterial Canker of Mango Diagnostic Methods.pptx

Bacterial canker of mango, caused by the bacterium Xanthomonas citri subsp. mangiferae, poses a significant threat to mango cultivation worldwide. Diagnosing this disease accurately is a pivotal step in effectively managing its impact on orchards. A range of diagnostic methods is employed to identify the presence of the pathogen and confirm the disease's occurrence. These methods encompass both visual observations and sophisticated laboratory techniques, collectively providing a comprehensive understanding of the disease's prevalence and severity. Through these diagnostic measures, farmers and agricultural experts can make informed decisions to mitigate the disease's effects and safeguard mango production.

1. Bacterial Canker of Mango – Diagnostic Methods

2. Symptoms of Bacterial Canker Xanthomonas campestris pv . mangiferaeindicae

3. Common Methods Used in Diagnosis Visual Examination Culture -dependent Methods Serological Methods Examine for presence of symptoms such as Detect bacterial proteins in plant tissue using leaf spots, stem cankers, and fruit lesions, which are indicative of bacterial canker disease. isolate and identify the pathogen. Antibody-based ELISA tests to identify bacterial canker pathogens. Grow bacteria obtained from diseased tissue on selective media supplements to

4. Enzyme-linked Immunosorbent Assay (ELISA) ELISA uses specific antibodies to detect bacterial proteins present in diseased host tissues and can yield results within a few hours. Antibodies that bind to the bacterial antigens are added to a sample of the diseased tissue. If bacterial canker is present, the antibody will bind to the antigen, producing a color change that can be measured spectrophotometrically. The final result of ELISA is a measure of the amount of bacterial antigen present in the sample, which is used to confirm the presence of bacterial canker.

5. Challenges in Diagnosis Symptom Similarity Sampling Timing The time of sampling during the disease development stage can affect diagnostic accuracy, making it essential to sample at the optimal time. Limited Compatibility Current methods are often not compatible for detecting multiple bacterial infections or the pathogen in a mixed infection, which can lead to challenges in diagnosis . Many bacterial diseases of mango have similar symptoms, making it difficult to differentiate bacterial canker from other diseases.