Bacterial community composition of Fungus .pptx

Bacterial Community Composition in the growth process of Pleurotus eryngiii. It is an edible fungus

1. Bacterial Community Composition in the Growth Process of Pleurotus eryngii and Growth-Promoting Abilities of Isolated Bacteria(Original Article) RAFIA ANJUM ID:2022764

2. Introduction Pleurotus eryngii is an edible mushroom. Bacterial community helps to improve nutrition uptake, promote the growth of host fungi , prevent pathogen contamination. Aim of the Research: The aim of this study is to investigate the bacterial community in different growth stages(Vegetative and Reproductive growth phase) of Pleurotus eryngii by high-throughput sequencing technology to explore the relationship between interacting bacteria and the growth and development of Pleurotus eryngii

3. Materials and Methods Sample collection: Samples were collected from different growth stages. It was mixed and frozen by liquid nitrogen. 2.DNA Extraction: DNA was extracted by Easy pure Genomic kit . 16s rDNA was amplified targeting V3 and V4 region. 3. High –Throughput Sequencing: Amplifications were sequenced by Illumina Hiseq and OTUs were assigned into taxonomic categories . 4. Cell Free Fermentation Broth Assay: The growth speed was analyzed by adding cell free fermentation broth.

4. Result Analysis Figure :1 Chord diagram visualizing relative abundances of the dominant bacterial phyla and genera in different growth stages of P. eryngii . The ten most abundant phyla and the genera with relative abundances of > 1% were showed.

5. Result Analysis Figure 2:Mycelia growth rate of P. eryngii cultured in media with and without the different volumes of B. cereus Bac1 fermentation broth.

6. Discussion: Ten abundant phyla with 14 abundant genera being shared across all growth periods. The results showed that when a 5 mL cell free fermentation broth was used, the growth speed of P. eryngii hyphae was enhanced.

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