BC-5300(new)&BC-5380(new)_Liquid System_V1.0_EN.pptx

Sistema de liquidos

1. BC-5300(new)/5380(new) Service Training Hematology Global Technical Support Department

2. Contents Product Overview Counting Principle Hardware System Mechanical System Installation Software System Liquid System

3. Counting Principle Review Diff Channel Laser + FCM WBC/HGB Channel Electrical Impedance Method Colorimetric Method RBC/PLT channel Electrical Impedance Method

4. Liquid Sequence —— Key points Drive power (e.g.: syringe, liquid pump etc.) Start point (e.g.: lyse bottle, counting bath etc.) End point (e.g.: counting bath, waste container etc.) Path (e.g.: start point → SV1 → LP → end point ） Volume (e.g.: 100uL) SV1 SV3 SV2 LP schematic Syringe

5. Liquid Sequence —— Sample aspiration （ CBC+DIFF ） 9uL : DIFF bath 6uL: WBC/HGB bath 5ul drain out Air Diluent 40uL: DIFF bath 40uL: WBC/HGB bath Air Diluent 180ul diluent 20ul blood Whole blood mode Pre-diluted mode

6. Liquid Sequence —— Sample aspiration （ CBC ） 6uL : WBC/HGB bath 9ul drain out Air Diluent 40uL: WBC/HGB bath Air Diluent 180ul diluent 20ul blood Whole blood mode Pre-diluted mode

7. Liquid Sequence —— Sample distribution

8. Liquid Sequence —— Reagent Volume Required Whole blood mode

9. Liquid Sequence —— Reagent Volume Required Pre-diluted mode

10. Liquid Sequence —— Hydraulic Diagram Sample Aspiration WBC Channel RBC Channel DIFF Channel Lyse Syringes Pressure & Vacuum

11. Liquid Sequence —— Sample Aspiration

12. Liquid Sequence —— Lyse Syringes

13. Liquid Sequence —— Pressure & Vacuum

14. Liquid Sequence —— DIFF Channel Sample Reaction Pre-Cleaning Sample Preparing Sheath Fluid Formation Auxiliary Dispensing Counting

15. Liquid Sequence —— DIFF Channel Cleaning

16. Liquid Sequence —— WBC/HGB Channel Sample Preparing Counting

17. Liquid Sequence —— WBC/HGB Channel Cleaning

18. Liquid Sequence —— RBC/PLT Channel

19. Liquid Sequence —— Start up

20. Liquid Sequence —— Shut down and Pack up

21. Version Version Author Date Description 1.0 Tata Ren 13/AUG/2014 BC-5300(new)/BC-5380(new) Liquid System First Release

22. Verification Version Verifier Date Comments 1.0 Mars Zhang 13/AUG/2014 Approved