BENDICT,S TEST.pptx

bendict,s test in urine sugar
chemical examination of bendict,s test

1. SURENDRA SIR B MLT-2020-24 CHEMICAL EXAMINATION BENEDICT’S TEST

2. DETERMINATION OF GLUCOSE OR SUGAR IN URINE. OBJECTS:- BENDICTS QUALITATIVE TEST REQUIREMENTS: - Test tube Test tube holder Burner Benedicts Regents. Dropper

3. PRINCIPLE:- The test is based on the reduction of alkaline blue cupric sulphate to yellow or red cuprous oxide by the aldehyde or ketone group of carbohydrates . in the absence of reducing substances in urine. The color of the reagent remains blue-green.

4. PRODECURE: - *Take 5 ml of benedict’s regent in test tube *Add 0.5 ml (8 drops) of Urine *Mix well and Boil 2-5 min. *Cool IN TAP WATER and note the color

5. OBSERVATION:- color RESULT- Green colour -Negative Yellow -green precipitate-+ Greenish -Yellow precipitate-++ Yellow precipitate-+++ Orange to brick -red -++++

6. CLINICAL SIGNIFICANCE :- Presence of glucose in Urine is found in Diabetes mellitus Hyperthyroidism Hyperpituitarism Disease of pancreas Renal glycosuria