Bioline lab PRESENTATION.pptx das das .

This a report presentation for all students. What you do tha training days and explain the process.

1. www.vanetragroup.in Presented By PRANOWDAS. K (22UBT030) VIDYAPATHI. R (22UBT045) YUVASURYA. K (22UBT050) POOVENTHIRAN. S (22UBT029) Under guidance of Mrs. K. Chitra, M.Sc., M.Phil Mr. K. Mahendran Assistance professor Pre analytical manager Department of Biotechnology Bioline Laboratory MCAS (Autonomous) 44\3, Cowley brown road, R asipuram , Namakkal-637408 R.S Puram , Coimbatore – 641002 PH: 8220042052 INTERNSHIP REPORT

2. www.vanetragroup.in Introduction What is your internship work? Haematology Microbiology Serology Clinical Biochemistry

3. www.vanetragroup.in HAEMATOLOGY Hematology is the study of blood and blood disorders. Hematologists and hematopathologists are highly trained healthcare providers. They specialize in diseases of the blood and blood components. These include blood and bone marrow cells. AIM: The Anti-A, Anti-B, and Anti-A,B reagents are used in the red blood cell determination of the ABO blood group. They are used to determine the absence or presence of erythrocytic antigens A and/or B on the surface of human red blood cells.

4. PROCEDURE 1.Add three drops of blood in a clean glass slide 2.Add antisera A, B and D sequentially to the 1st, 2nd and 3rd drop of blood 3.Properly mix the antisera with the blood by separate toothpicks 4 . Allow to stand for 2-3 minutes and note down the result on the basis of clump formation

5. RESULT ANTI A ANTI B ANTI D BLOOD GROUP + _ + A POSITIIVE _ + + B POSITIVE _ _ + O POSITIVE + + + AB POSITIVE + _ _ A NEGATIVE _ + _ B NEGATIVE _ _ _ O NEGATIVE

6. SEROLOGY Serology is the scientific study of serum and other body fluids. In practice, the term usually refers to the diagnostic identification of antibodies in the serum . Syphilis Ab Rapid Test – Cassette AIM: TRUSTline Syphilis Ab Rapid Test – Cassette for the qualitative detection of antibodies to Treponema pallidum ( Tp ) in Human Serum / Plasma.

7. TRUSTline Syphilis Ab Rapid Test - Cassette

8. www.vanetragroup.in PROCEDURE Step 1: Bring the specimen and test components to room temperature if refrigerated or frozen. Mix the specimen well prior to assay once thawed. Step 2: When ready to test, open the pouch at the notch and remove the device. Place the test device on a clean, flat surface. Step 3: Be sure to label the device with the specimen ID number. Step 4: Fill the specimen transfer device with the specimen. Holding the specimen transfer device vertically, dispense 2 drops (about 60-90 µL) of specimen into the sample well, making sure there are no air bubbles. Note: Add 1 drop of Saline or Phosphate-Saline buffer (common buffers used in clinics not provided in the kit) to the sample well if flow migration is not observed in the result window within 30 seconds, which could occur with highly viscous specimens. Step 5:Set up timer. Step 6: Results can be read at 15 minutes. Positive results may be visible in as soon as 1 minute.Do not read result after 20 minutes. To avoid confusion, discard the test device after interpreting the result.

9. www.vanetragroup.in INTERPRETATION OF ASSAY RESULT 1. NEGATIVE RESULT: If only the C line is developed, the test indicates that no detectable anti- Tp antibody is present in the specimen. The result is negative or non- reactive. 2. POSITIVE RESULT: If both the C and T lines are developed, the test indicates the presence of anti- Tp antibodies in the specimen. The result is positive or reactive. Specimens with positive results should be confirmed with alternative testing method(s) and clinical findings before a diagnostic decision is made. 3. INVALID: If no C line is developed, the assay is invalid regardless of color development on the T line as indicated below. Repeat the assay with a

10. RESULT

11. MICROBIOLOGY STREAK-PLATE TECHNIQUE Aim :The streak-plate procedure is designed to isolate pure cultures of bacteria, or colonie . Materials required : Blood agar and mackkon key agar plate Inoculation loop Patients blood or urine sample Disposal gloves and mask

12. PROCEDURE Step 1 : Sterilize the inoculating loop . Step 2 :Touch the loop to the agar surface to cool it down. .. Step 3 :Inoculate the loop by dipping it into broth, or touching it to the surface of a bacterial colony Step 4 : after , incubate the plates 24 hour's and results will be seen

13. GRAM’S STAINING Aim : A Gram stain is most often used to find out if you have a bacterial infection Materials required : 1.Primary stain : crystal violet 2.Grams iodine 3.Decolourizer ethanol 95 % 4.Counter stain : safranin

14. PROCEDURE Step 1 :Prepare a thin smear on clear, dry glass slide . Step 2 :Allow it to air dry and fix by gentle heat . Step 3 :Flood with Gram's Crystal Violet for 1 minute. (If over staining results in improper decolourization of known gram negative organisms, use less crystal violet ). Step 4 :Wash with tap water . Step 5 :Flood the smear with Gram's Iodine. Allow it to remain for 1 minute . Step 6 : Decolourize with Gram's Decolourizer until the blue dye no longer flows from the smear. (Acetone may be used as a decolourizing agent with caution, since this Step 7 :solvent very rapidly decolourizes the smear ). Step 8 :Wash with tap water . Step 9 :Counter stain with 0.5% w/v Safranin Allow it to remain for I minute.Step 10 :Wash with tap water.Step 11 :Allow the slide to air dry and examine under oil immersion objective

15. RESULT

16. CONCLUSION What are the techniques you learned, just summarize it

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