biosafety-150427104507-conversion-gate01.pptx

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1. Biosafety levels

2. Hazards in the lab Biological : exposure to blood and body fluids and specimens that harbour HIV etc., bioterrorism) Chemical : acids, alkalies , toxic chemicals Radiological : ineffective radioactive waste disposal Fire Electrical accidents

3. Protection: workers products co-workers lab support personnel environment Why Biosafety Practices? Introduction

4. Safety Documentation & Records Laboratory Safety Manual - Policies and Procedures Sample Contents: Housekeeping Personal protection Safe decontamination of equipment Decontamination & Waste Disposal Emergency procedures In-lab first aid Accidental injury Post exposure prophylaxis Contacts Personnel responsibilities Hygiene

5. Safety Resources

6. Action Plan for Implementing Safety Practices Identify hazards Assess level of risks Prioritize risk Establish and implement safety polices and procedures Conduct safety specific training Must be a priority Communication is key Perform regular audits and assessments

7. Biosafety is the application of safety precautions that reduce a laboratorians risk of exposure to a potentially infectious material and limit contamination of the work environment and ultimately the community { CDC- Centers for Disease Control and Prevention }

8. Why we need biosafety ???? Lab has hazards of processing infectious agents Accidental threat to workers and environment To have adherence with safety regulations while dealing with highly infectious agents

9. BIOSAFETY LEVEL 1 Microbes not known consistently to cause disease in healthy adults and present minimal potential hazard to lab and environment Eg : non pathogenic strain of E.coli

10. BSL – 1 practices: Standard microbiological practices are followed Work can be performed on an open table or bench Sink – hand washing Lab – doors seperate

11. Biosafety level 2 Microbes that possess moderate hazards to laboratorians Eg : Staphylococcus aureus

12. BSL – 2 practices: Access to lab is restricted when work is being conducted PPE , face shields, eye goggles Biosafety cabinet Autoclave/Decontamination proper Self closing doors Sink with eyewash apparatus readily available

13. Biosafety level 3 Serious / potentially lethal disease through respiratory transmission Eg : Mycobacterium tuberculosis

14. BSL 3 - practices Laboratorians – under medical surveillance and receive immunisation Access to lab restricted & controlled PPE with respirators BSC Sink with eyewash Exhaust air – not recirculated Self closing doors with automatic locking

15. Biosafety level 4 Highest level of biological safety Dangerous and exotic microbes Eg : Ebola virus

16. BSL 4

17. BSL 4 - practices Change clothes before entering Shower upon exiting Decontaminate all materials before exiting Class III BSC Separate building for lab Vacuum lines and decontamination systems

18. BSL 4 lab

19. Thank you……….