Biosurfactant

COMPARATIVE STUDY OF BACTERIA FOR BIOSURFACTANT PRODUCTION BY USING DIFFERENT CARBON SOURCES

1. COMPARATIVE STUDY OF BACTERIA FOR BIOSURFACTANT PRODUCTION BY USING DIFFERENT CARBON SOURCES BY: -ABHISHEK M SIRSIKAR -SAMEER S. HADAWALE -ANSHULKUMAR GUPTA VIT UNIVERSITY, VELLORE

2. Synopsis : Introduction Importance of biosurfactant in bacteria Pathways for biosurfactant production Objective Experimental Details Applications

3. Introduction Surfactants both chemically and biologically are amphiphilic compounds. Biosurfactants possess different chemical structures--lipopeptides, glycolipids, neutral lipids and fatty acids. They are nontoxic biomolecules that are biodegradable.

4. Importance of biosurfactant in bacteria The low water solubility of these hydrophobic compounds limits their availability to microorganisms. They also have vital importance to the microbes in adhesion, emulsification, bioavailability, desorption and defense strategy. It plays an essential role in the swarming motility of microorganisms and participate in cellular & physiological processes of signalling and differentiation as well as in the biofilm formation. It have antibacterial and antimicrobial property.

5. Pathways for biosurfactant production:

6. Examples of biosurfactant

7. Objective Our moto is to identify the efficient biosurfactant producing bacteria under controlled supply of different Carbon sources. And see the ability of bacteria how much yield it produced by giving different carbon sources such as dextrose , yeast,beef and peptone.

8. Screening Isolation and culturing Inoculum Preparation Inoculation and Incubation Extraction Qualitative Analysis Experimental Details

9. Tests Oil Displacement Emulsification Index

10. Hemolytic Activity Biosurfactant Yield

11. Agriculture (pest control, biofertilizers ) Washing, hygienic, cosmetic means Inhibition of corrosion Pharmaceutical industry Food industry Bioremediation of soils and water Applications:

12. The directions of R&D Investigation of synthesis of biosurfactants , their properties and application in biomedicine, agriculture, food, pharmaceutical and cosmetic industry. Development of methods of biotechnological remediation of water and soil Biosynthesis and studying of microbial enzymes and their compositions Creation and investigation of new polymeric materials Monitoring of petroleum-contaminated water and soil

13. Thank You very much!