Blast

Bioinformatics Tools

1. Presentation On BLAST P R E S E N T E D B Y Nagesh Murge

2. Definition Introduction NCBI home page NCBI-BLAST home page EMBL-EBI BLAST page BLAST Input & Output Type of BLAST BLAST Function Objectives of BLAST Contents

3. Definition The Basic Local Alignment Search Tool (BLAST) for comparing gene and protein sequences against others in public databases. BLAST is a set of sequence comparison algorithms used to search databases for optimal local alignments to a query.

4. Today , one of the most commonly used tools to examine DNA and protein sequences is the Basic Local Alignment Search Tool, also known as BLAST . BLAST is a computer algorithm that is available for use online at the National Center for Biotechnology Information (NCBI) website and many other sites Introduction

5. NCBI Home Page

6. NCBI-BLAST Home Page

7. EMBL-EBI BLAST Page

8. Input Input sequences In FASTA or Genbank format. Output BLAST output can be delivered in a variety of formats. These formats include HTML,plain text, and XML formatting . For NCBI's web-page, the default format for output is HTML BLAST -Input & Output

9. Nucleotide-nucleotide BLAST ( blastn ) Compares nucleotide query against nucleotide sequence database Protein-protein BLAST ( blastp ) Compares protein query against proteins sequence database. Types of BLAST

10. Large numbers of query sequences ( megablast ) : When comparing large numbers of input sequences via the command-line BLAST, " megablast " is much faster than running BLAST multiple times . Of these programs,BLASTn and BLASTp are the most commonly used because they use direct comparisons, and do not require translations. Types of BLAST

11. BLAST can be used for several purposes. These include : I dentifying species, locating domains , E stablishing phylogeny, DNA mapping, C omparison . BLAST Function

12. It is one of the most popular programs for sequence analysis . Enables a researcher to compare a query sequence with a library or database of sequence . Alignments of the high-scoring segment pairs showing identical and similar residues . Objectives of BLAST