CAMAG Planar Chromatography with visionCATS

PPT Camag OLD

1. Planar Chromatography
with visionCATS

2. Why visionCATS – the concept

3. HPTLC today
Suitable instruments / Software
Application
Development
Derivatization
Documentation
Evaluation
A new concept
Scientific basis
Standardized methodology
Validated methods
Qualified data
 Global collaboration
High Performance Thin-Layer Chromatography
Planar chromatography of the
21
st
century
 Plug and play …

4. What you need:
na method
nTLC or HPTLC precoated layers
nsuitable equipment
nknow how
Planar Chromatography...

5. nA method...
Developed and
validated by
CAMAG
Planar Chromatography...

6. Application Notes

7. Sources of Methods
Links relevant for Herbals:
nhttp://www.botanicalauthentication.org/index.php/Category:HPTLC 
(APHA)
nhttp://hmc.usp.org/monographs/all (USP Herbal Medicine Compendium)
nhttp://www.hptlc-association.org/methods.cfm (HPTLC Association)
General methods:
nhttp://www.camag.com/en/tlc_hptlc/camag_laboratory/methods.cfm?ao=-1
(CAMAG Application notes)
nhttp://www.camag.com/en/tlc_hptlc/customer_magazine_cbs/ccbs_database.cfm
(CCBS database)

8. Case Studies

9. www.camag.com

10. Case Study - Example

11. Download methods for free...
www.camag-laboratory.com

12. Youtube and Labtube
nVisit our channels!
Product videos: ATS 4, ADC 2, Derivatizer, visionCATS
Application tutorials: Natural products, Food, Industrial and Forensic applications
Visit our channels!

13. CAMAG CBS
nCAMAG Customer Magazin: Application
examples from different fields
nCumulative CAMAG Bibliography Service:
A collection of abstracts of publications in
the field of Thin-Layer Chromatography

14. Request for Laboratory Services

15. Application Fields (Markets)
Clinical
Applications
Food and Feed
Pharmaceutical
Applications
Herbals medicines
Cosmetics
Industrial
Applications
Forensics
Environment

16. What you need :
na method
nTLC or HPTLC precoated layers
nsuitable equipment
nknow how
Planar Chromatography...

17. nTLC/HPTLC precoated layers
…available at CAMAG or at
your lab supplier
Planar Chromatography...

18. TLC vs. HPTLC
TLCHPTLC
Mean particle size:10 - 15 µm5 - 7 µm
Particle distribution:widenarrow
Layer thickness:250 µm 100, 200 µm
Number of samples:max. 1236 - 72
Migration distance:100 - 150 mm30 - 70 mm
Migration time: 30 - 200 min 3 - 20 min
Solvent use:50 ml 5 - 10 ml
Detection limit: Abs100 - 1000 ng10 - 100 ngFluor1 - 100
ng0,1 - 10 ng

19. Comparison TLC-HPTLC
Flavonoids (Ph.Eur.4)
HPTLC plate 20 x 10 cm
7 cm migration
10 mL solvent
10 min.
TLC plate 20 x 20 cm
12 cm migration
25 mL solvent
32 min.

20. What you need:
na method
nTLC or HPTLC precoated layers
nsuitable equipment
nknow how
Planar Chromatography...

21. Planar Chromatography
SampleSample applicationapplication
ChromatogramChromatogram developmentdevelopment
Detection & quantitative evaluationDetection & quantitative evaluation
DocumentationDocumentation
Post-Chrom. DerivatizationPost-Chrom. Derivatization

22. Planar Chromatography
nSample application is the most critical step in the
TLC procedure.
Errors which occur during sample application,
cannot be compensated for in chromatogram
development or evaluation.
SampleSample applicationapplication

23. step by step
nSample Application
Planar Chromatography...

24. Sample ApplicationSample Application
Nanomat
volume volume volume
1µL
Linomat
Spray-on technique
2µL 1µL 2µL 10µL5µL
Spot versus Band applicationSpot versus Band application

25. Sample application - semi automatic LINOMAT5
nSprays on samples as narrow bands
nLarger volumes can be applied (1 - 200 L)
nPrecise sampling of programmed volumes
nSample application for:
•qualitative
•quantitative
•preparative separation
nLINOMAT

26. Linomat 5
Keypad & Display
Plate holder
Turret with dosing syringe

27. Sample application: fully automatic ATS4
nPC - controlled
nApplication of spots, bands or rectangles
nReduced time for application
nImproved reproducibility
nSuitable for danger substances

28. Automatic TLC Sampler 4
Display
Rack for sample vials
or micro-titer plates
Turret with dosing syringe
Protection cover Waste bottle
Rinsing bottle
Plate holder

29. Automatic TLC Sampler 4
Contact transfer = Spots
Spray-on = Bands
Spray-on = rectangular

30. step by step
nSample Application
nChromatogram Development
nEvaluation
nDocumentation
Planar Chromatography...

31. Chromatogram development
nFully automatic gradient separation via AMD2
nManual separation via Glass tank
nAutomatic isocratic separation via ADC

32. ADC2
Chromatogram development
with standardized conditions in a
conventional glass tank
n fully automatic
n saturation controlled
n humidity controlled
n qualifiable IQ/OQ

33. ADC 2 Reproducibility
(triamteren / rhodamine)
nSame results day after day

34. Derivatization
Dipping
nUniform layer coverage
nProduces tide mark
nRigorous procedure
required
nHigh background
coloration
Spraying
nForm of art
nLittle reagent
consumption
nMay form hazardous mist
nReproducibility is a
problem

35. TLC -sprayer

36. Chromatogram Immersion Device III
Derivatization by dipping
nUniform Reagent transfer
nReproducible results
nAllows standardized procedure
nImmersion time and vertical
speed selectable

37. step by step
nSample Application
nChromatogram Development
nEvaluation
nDocumentation
Planar Chromatography...

38. Visual inspection in Viewing box

39. Densitometry
TLC Scanner 4

40. Image and profile (densitogram)
A
b
s
o
r
p
t
i
o
n
[
A
U
]
Start
Migration distance [mm]

41. Densitometry

42. visionCATS - Spectrum

43. Spectrum Scan
Display of the selected spectra

44. Detection – multi wavelength scan
A selection of up to
31 WL is possible

45. step by step
nSample Application
nChromatogram Development
nEvaluation
nDocumentation
Planar Chromatography...

46. TLC VISUALIZER:
Documentation, Evaluation, Archiving

48. TLC Visualizer
nDesign has been enhanced to further improve the
homogeneity of the illumination of the plate:
•254 nm, short wave UV- Reflectance
•366 nm, long wave UV - Reflectance
•White light - Reflectance and/or
Transmission.
nErgonomic design: Easy access in to the illumination
compartment via large sliding door.
nseparate viewing window with UV-protection
nTimer for automatic switch-off of the light sources

49. Automatically finding the brightest spot (UV 366 mn)
Automatic Optimization

50. Manual Optimization
If desired, a region of interest can be defined, in which the brightest spot is
automatically found.

51. visionCATS – Image Comparison
nImage Comparison – fully integrated
> 40 samples of
Curcuma
(plates 1 – 3)

52. visionCATS – Image Comparison
nImage Comparison – fully integrated
Image Comparison – fully integrated

53. visionCATS – Image Comparison
nImage Comparison – fully integrated
Image Comparison – fully integrated

54. Visualizer together with visionCATS

55. visionCATS - Data View & Image Profile

56. Image enhancement: “Clean Plate”

57. Image enhancement: “SpotAmp”
Contrast 1.0Contrast 1.4Contrast 2.0Contrast 2.8Contrast 4.0

58. Image enhancement: exposure “normalization”
Comparing chromatography from 3
different plates

59. Image enhancement: “Clarify”

60. visionCATS - Method Library

61. Import of methods from the Method Library

62. Advanced image evaluation
Pixels
R: 223;
G: 160;
B: 003;
For each R
F value the mean luminance
of the pixels (red, green, blue) in the
zone is calculated
Y = 0.3 R + 0.59 G + 0.11 B
Calculated on an aliquot of
the track (50 %)
The combination of all
generates a profile

63. visionCATS - The idea
Sample application
Development
Detection & Evaluation
Documentation
Derivatization
One software to manage all
steps of Planar
Chromatography

64. … is GLP/GMP compliant:
All significant parameters of
a TLC method are recorded
and electronically stored.
Planar Chromatography...

65. What you need:
na method
nTLC or HPTLC precoated layers
nsuitable equipment
nknow how
Planar Chromatography...

66. nKnow How...
Application Lab
Training courses
individual Training
Planar Chromatography...

67. Why CAMAG?
n> 40 years of experience
nsatisfied customers
nindividual solutions
ncompetent After Sales Service
ntraining courses
Planar Chromatography...

68. CAMAG worldwide
nCAMAG Muttenz / Switzerland
www.camag.ch
nCAMAG China Supporting Unit Hong Kong & Guangzhou
www.camag-china.com
nCAMAG Berlin / Germany
www.camag-berlin.de
nCAMAG Scientific Inc. North Carolina / USA
www.camagusa.com
nCAMAG Regional Support Unit Bombay / India
www.hptlc-anchrom.in

69. ..... no laboratory is ..... no laboratory is
complete without a complete without a
modern HPTLC system!modern HPTLC system!
Conclusion ...Conclusion ...

70. Thanks for your attention !