Cbc coulter principle

Cbc coulter principle

1. CBC- Coulter Principle
Caesar Abu Arra
MSc Life sciences
BSc Medical lab. Sciences
Medicare Labs

3. Electrical impedance: Coulter principle

The sample is divided into two aliquots and
mixed with an isotonic diluent:
1-
The first dilution is delivered to the RBC aperture bath:
RBCs and the platelets are counted and discriminated by
electrical impedance
Particles between 2 and 20 fL are counted as platelets.
Those greater than 36 fL are counted as RBCs.

4. Electrical impedance: Coulter principle


2-
The second dilution is delivered to the WBC aperture bath:
Lysing reagent is added to the WBC dilution to lyse RBCs 
release hemoglobin (before the WBCs are counted)
WBCs are counted by electrical impedance.
 The WBC dilution is passed to the hemoglobinometer for
hemoglobin determinationlight transmittance at a
wavelength of 535 nm.
The analyzer automatically cleans the remaining blood by
cleaning reagent.

7. THANK YOU
ABU JAD CAESAR