Charles Proposal Presentation escher.pptx

charli

1. Title: THE ANTIMICROBIAL-RESISTANT STRAINS OF PATHOGENIC ESCHERICHIA COLI ISOLATED FROM NAKURU COUNTY ABATTOIR WASTEWATERS Presented By: Charles Ndungu Kibuthu

2. Introduction 2 Global Meat Consumption Increase: 40% rise leading to 2021 Impact on Waste Production: Increase in meat-processing waste Public Health Concern: Pathogenic waste poses risks to humans, animals, and the environment

3. Research Problem Abattoir waste harbors enteric pathogens Enteric infections from abattoir wastewater Antibiotic-resistant E. coli pathotypes A point source of antimicrobial-resistant pathogens 3

4. Research Problem Abattoir Waste Composition: Includes wastewater, waste meat, fecal material Public Health Burden: Harbors pathogenic enteric microbes, risk of antibiotic resistance Need for Sustainable Management: Effective waste treatment is crucial 4

5. Questions What is the occurrence of E. coli in Nakuru county abattoir wastewaters? What is the antimicrobial sensitivity profile of these E. coli isolates? What are the prevalent pathotypes of antimicrobial-resistant E. coli in these wastewaters? 6

6. Objectives General Objective Determine the antibiotic-resistant profiles of pathogenic E. coli from abattoir wastewaters. Specific Objectives Isolate and identify E. coli from wastewater samples. Characterize antibiotic resistance profiles. Classify antimicrobial-resistant E. coli based on virulence genes. 7

7. Justification of the Study Urgent Need: Rising demand for meat leads to more contaminated waste Public Health Risk: Potential outbreaks of antimicrobial-resistant infections Study Aim: Investigate pathogenic E. coli presence and antibiotic sensitivity profiles 8

8. Methodology Study Site: Nakuru County, Kenya Sample Collection: sample 67 abattoirs Isolation and Identification: Cultural methods and PCR-based molecular assays Antibiotic Sensitivity Testing: Kirby-Bauer disk diffusion method 10

9. Expected Results Isolation of E. coli: From abattoir wastewater samples Antibiotic Resistance Profiles: Detailed sensitivity profiles of isolated strains Pathogenic Strains: Identification of antimicrobial-resistant E. coli strains 11

10. Significance of the Study Understanding Resistance Dynamics: Insight into antibiotic resistance in abattoir wastewater Public Health Recommendations: Inform strategies to prevent antimicrobial-resistant infections Further Research: Basis for national prevalence studies 12

11. Conclusion Summary: Rising meat consumption increases waste and public health risks Importance of Study: Critical to address antibiotic resistance in E. coli from abattoir wastewaters Future Steps: Develop and implement effective waste management and decontamination strategies 13

12. THANK YOU Presented By : Charles Ndungu Kibuthu

13. References Yamane, Y. (1967). Mathematical formulae for sample size determination.  J. Mathemetics ,  1 , 1-29.