Clinical laboratory technology- Basic laboratory principles

Basic laboratory principles
Accuracy and precision

1. VIVEKANANDHA ARTS AND SCIENCE
COLLEGE FOR WOMEN VEERACHIPALAYAM
– 636007,SANKAGIRI,TAMILNADU
DEPARTMENT OF MICROBIOLOGY
SUBJECT – CLINICAL LABORATORY TECHNOLOGY
TOPIC – BASIC LABORATORY PRINCIPLES
SUBJECT INCHARGE :
DR.V.BHARATHI
ASSISTANT PROFESSOR
DEPARTMENT OF
MICROBIOLOGY
VIAAS, SANKAGIRI
PRESENTED BY :
S.ANISHA
ll- B.Sc MICROBIOLOGY
DEPARTMENT OF
MICROBIOLOGY
VIAAS, SANKAGIRI

2. https://en.wikipedia.org/wiki/Laboratory
BASIC LABORATORY
PRINCIPLES

3. CONTENT
• Introduction
•Safety first
•Accuracy And Precision
•Proper documentation
•Good equipment design
•Equipment handling
•Sterile techniques
•Ethical content
•Conclusion

4. INTRODUCTION
Basic laboratory principles form the foundation for conducting safe, accurate,
and effective scientific experiments. Whether in chemistry, biology, or physics,
these principles ensure that experiments are carried out methodically, with
careful attention to detail and safety. They emphasize the importance of proper
safety procedures, accurate measurements, precise documentation, and ethical
conduct. By following these guidelines, scientists and students alike can
minimize risks, prevent contamination, and produce reliable, reproducible
results. Understanding and applying these core principles is essential for success
in any laboratory environment

5. SAFETY FIRST
Personal Protective Equipment (PPE):
Always wear appropriate PPE like lab coats, gloves, goggles, and masks.
Chemical Safety:
Understand the properties of chemicals, including hazards like toxicity, flammability, and corrosiveness.
Always follow Material Safety Data Sheets (MSDS) for guidance.
Proper Disposal:
Dispose of chemicals, biological waste, and broken glassware according to the lab’s safety protocols.
Emergency preparedness:
Knows now the location of emergency exits, fire extinguishers, eye wash stations, and first aid kits.

6. IMAGE

7. ACCURACY AND PRECISION
Measuring instrument:
Use calibrated instruments for accurate measurements (e.g.,
balances, pipettes, burettes)
Repetition and Consistency:
Repeat experiments to ensure consistent and reliable data.
Avoid Contamination:
Always use clean equipment to prevent cross-contamination,
especially in microbiological work.

8. PROPER DOCUMENTATION
Lab Notebook:
Maintain a detailed and organized lab notebook,
recording procedures, observations, and results.
Labeling:
Clearly label all containers, solutions, and samples with
relevant information (e.g., chemical name,
concentration, date).

9. GOOD EXPERIMENTAL DESIGN
Control groups:
Use controls to compare with experimental groups and
validate the results.
Reproducibility:
Ensure that experiments can be repeated under the
same conditions for consistent results.
Variables:
Clearly define dependent, independent, and controlled
variables to guide your experimental setup

10. EQUIPMENT HANDLING
Proper Use:
Follow instructions for using lab equipment such as microscopes,
centrifuges, and spectrophotometers.
Calibration:
Regularly calibrate instruments to ensure they provide accurate
readings.
Maintenance:
Keep equipment clean and well-maintained to avoid malfunctions
during experiments.

11. STERILE TECHNIQUES
Aseptic Conditions:
In biological experiments, especially microbiology,
practice aseptic techniques to avoid contamination by
microbes.
Autoclaving:
Use autoclaves to sterilize equipment and culture
media

12. ETHICAL CONDUCT
Honesty in Data:
Record all data truthfully and avoid manipulating results.
Respect for Life:
Follow ethical guidelines for handling living organisms,
ensuring minimal harm in experiments involving animals
or cell

14. CONCLUSION
In conclusion, adhering to basic laboratory principles is crucial for ensuring
safety, accuracy, and ethical responsibility in scientific research. These guidelines
not only protect individuals and the environment but also ensure that
experiments are reliable and reproducible. By practicing proper safety measures,
maintaining accurate documentation, and following ethical standards,
researchers can achieve high-quality results and contribute to the advancement
of scientific knowledge. A strong foundation in these principles fosters a
disciplined and efficient laboratory environment, enabling meaningful and
impactful discoveries.

15. REFERENCE
•https://en.wikipedia.org/wiki/Laboratory
•https://images.app.goo.gl/NiiHGq15vnBn4ayQ9
•https://images.app.goo.gl/7HKaa8BPPWsFpQux9
•https://images.app.goo.gl/rzNbzRoBGUaLrxx79

16. THANKYOU