Coags.pptx

Coags system

1. COAGULATION SYSTEM

2. Blood Coagulation The clotting mechanism involves a cascade of reactions in which clotting factors are activated. Most of them are plasma proteins synthesized by the liver (vitamin K is needed for the synthesis of factor II, VII, IX and X). They are always present in the plasma in an inactive form. When activated they act as proteolytic enzymes which activate other inactive enzymes. Several of these steps require Ca++ and platelet phospholipid.

3. Blood Coagulation The ultimate step in clot formation is the conversion of fibrinogen → fibrin.

4. Factor X can be activated by reactions in either of 2 systems: An Intrinsic system An Extrinsic system Once factor X has been activated, it complexes with calcium ions, PF3, and factor V to form prothrombin activator

5. Pathway to Thrombin Prothrombin activator catalyzes the transformation of prothrombin to the active enzyme thrombin

6. Common Pathways to the Fibrin Mesh Thrombin catalyzes the polymerization of fibrinogen into fibrin Insoluble fibrin strands form the structural basis of a clot Fibrin causes plasma to become a gel-like trap Fibrin in the presence of calcium ions activates factor XIII that: Cross-links fibrin Strengthens and stabilizes the clot

7. Intrinsic pathway The initial reaction is the conversion of inactive factor XII to active factor XIIa . Factor XII is activated in vitro by exposing blood to foreign surface (glass test tube). Activation in vivo occurs when blood is exposed to collagen fibers underlying the endothelium in the blood vessels.

8. The intrinsic pathway begins with the contact factors, factor XII, HMWK, and prekallikrein , which activates factor XI. Activated factor XI can then activate factor IX, which then acts with its cofactor,factor VIII,to form tenase complex on a phospholipid surface to activate factor X. Activated factor X then combines with its cofactor, factor V, to form the prothrombinase complex on a phospholipid surface, converting prothrombin to thrombin.

9. INTRINSIC SYSTEM

10. Extrinsic pathway Requires contact with tissue factors external to blood. This occurs when there is trauma to the vascular wall and surrounding tissues. The extrinsic system is triggered by the release of tissue factor (thromboplastin from damaged tissue), that activates factor VII. The tissue thromboplastin and factor VII activate factor X.

11. Tissue Factor Pathway Net result: Production of IXa Production of small amounts of thrombin ( IIa )

12. EXTRINSIC SYSTEM

13. Clot retraction Clot formation is fully developed in 3-6 min Contraction of platelets trapped within the clot shrinks the fibrin meshwork pulling the edges of the damaged vessel closer together. During clot retraction serum is squeezed from the clot.