Cobas device Electrochemiluminescence Immunoassays

Manufacture by Roche company [Switzerland ]
And Hitachi company [ Japanese ]
Estimation : chemistry, immune, viruses, tumor marker, hormone and vitamin
The first version : Elecsys 1010 and the Elecsys 2010 and developed into Cobas C311, Cobas E411, Cobas 6000, and Cobas 8000 ..

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Cobas device
Manufacture by Roche company [Switzerland ]
And Hitachi company [ Japanese ]
Estimation : chemistry, immune, viruses, tumor marker, hormone and vitamin
The first version : Elecsys 1010 and the Elecsys 2010 and developed into Cobas C311,
Cobas E411, Cobas 6000, and Cobas 8000 ..
Technology of Cobas : Electrochemiluminescence Immunoassays [ ECLIA]

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Benefits
Easy to use
Random Access
88 Test/Hour
Qualitative and Quantitative both test can be run
ECL (Electrochemiluminescence)Technology
Sample volume required/Test 10 – 50 UI
Touch screen
Data base storage up to 2000 results
Sample Type : Serum , Plasma , Urine
Test Methods : Sandwich , Competitive , Titration


Wash Systems
Conditioning of electrode Sysclean ; Sodium Hypochloeite solution used for the
cleaning of the measuring cell (every two weeks )
Sysclean :increasing rising efficiency between pipetting steps preventing bacterial
growth
Syswash added to the distilled water container with dilution of 1+100

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Electrochemiluminescence detection:
The electrochemiluminescent label is a ruthenium complex (Ru(bpy). In the
electrochemical reaction (Ru(bpy) is first oxidized to Ru(bpy) at the surface of the
electrode. At the same time tripropylamine (TPA, component of ProCell) which is
present in excess, is oxidized to form the cation radical TPA+* which spontaneously
loses a proton. The powerful oxidant Ru(bpy) reacts with the free radical TPA*,
a strong reductant, to form the excited state of the ruthenium complex, Ru(bpy). This
returns to the ground state by emitting a photon at 620 nm and is then again available
for a new light-generating reaction cycle

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