Column Chromatography.pptx

Chromatography is a powerful and advanced techniques for separating mixtures. Many types of chromatographic techniques are known, such as paper, thin layer, column chromatography, each with its own strength and weakness.

Chromatography system in general have a stationary phase and a mobile phase.

In column chromatography both phases are placed in a column container, i.e. all the chromatographic operations are carried out using column.

Column chromatography in chemistry is a method using for the Identification, separation and purify individual chemical compounds from mixtures of compounds in the large amount.
Column Chromatography is a separation technique in which components of mixture is separated by using a glass column packed with stationary phase and liquid mobile phase flowing continuously through the column.

It is suitable for the physical separation of gram quantities of material. A solvent acts as the mobile phase while a finely divided solid surface acts as the stationary phase.

Usually a glass tube with a diameter from 1cm to 10cm and a height of 20 cm to 50cm with a tap at the bottom, is used for this purpose.
Depending upon the flow of solvent down, column chromatography may be separated into two categories.

Gravity column chromatography
If the solvent is allowed to flow down the column by Gravity, or downward process, it is known as gravity column chromatography .

Flash chromatography
If the solvent is forced down the column by positive air pressure , it is called flash chromatography.

1. Column Chromatography Bilawal Shaikh Lecturer, IPS PUMHSW

2. Contents Introduction Types of column chromatography Principle of column chromatography Instrumentation techniques of column chromatography Factors affecting column efficiency 2

3. INTRODUCTION Chromatography is a powerful and advanced techniques for separating mixtures . Many types of chromatographic techniques are known, such as paper, thin layer, column chromatography, each with its own strength and weakness . Chromatography system in general have a stationary phase and a mobile phase . In column chromatography both phases are placed in a column container, i.e. all the chromatographic operations are carried out using column. Column chromatography in chemistry is a method using for the Identification , separation and purify individual chemical compounds from mixtures of compounds in the large amount. 3

4. Column Chromatography is a separation technique in which components of mixture is separated by using a glass column packed with stationary phase and liquid mobile phase flowing continuously through the column. It is suitable for the physical separation of gram quantities of material. A solvent acts as the mobile phase while a finely divided solid surface acts as the stationary phase. Usually a glass tube with a diameter from 1cm to 10cm and a height of 20 cm to 50cm with a tap at the bottom, is used for this purpose. 4

5. Types of column chromatography Depending upon the flow of solvent down , column chromatography may be separated into two categories . Gravity column chromatography If the solvent is allowed to flow down the column by Gravity , or downward process, it is known as gravity column chromatography . Flash chromatography If the solvent is forced down the column by positive air pressure , it is called flash chromatography. 5

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7. Types of Column Chromatography 1. Based on the difference of the flow rates of the mobile phase, column chromatography can be of several types: 1. Gravity column chromatography 2. Flash column chromatography 2.1 Low and medium pressure column chromatography 2.2 Vacuum Liquid Chromatography (VLC ) 2.3 High Performance/Pressure Liquid Chromatography (HPLC ) 7

8. Low and medium pressure column chromatography: The movement of mobile phase is accelerated by using pumps that generate low or medium pressure. The increase in the flow rate shortens the time of separation. 8

9. Vacuum Liquid Chromatography (VLC): The movement of the mobile phase is accelerated by creating vacuum. The adsorbent is applied dry into a sintered glass funnel. The sample is applied by dry method or as solution. Then the mobile phase is added portion by portion and vacuum is applied after each portion to collect each fraction. 9

10. High Performance/Pressure Liquid Chromatography (HPLC): In this column very fine silica gel is used, so separation power is greatly increased. However, the flow rate of the mobile phase is severely decreased. High pressure pumps are used to push the solvent through the column which in this case must be made of stainless steel. 10

11. 2. Based on types of stationary phase and mobile phase: Liquid-solid chromatography: also known as column adsorption chromatography or classical column chromatography. Here the stationary phase is solid and the mobile phase is liquid. Liquid-liquid chromatography: also known as column partition chromatography or partition chromatography. Here both the stationary and mobile phase is liquid. 11

12. Principles of Column Chromatography Column Adsorption Chromatography Partition Chromatography Ion-Exchange Chromatography Size exclusion chromatography or Gel permeation chromatography Affinity Chromatography 12

13. Principle Of Column Chromatography Column adsorption chromatography: A solid stationary phase and a liquid mobile phase and is used and the principle of separation is adsorption. It is known that the rate of adsorption varies with a given adsorbent for different materials. Generally , in this techniques , the mixture to be separated is taken in suitable solvent and small amount of solution is added to the uniformly packed column with adsorbent. The sample is allowed to the enter. A suitable developing solvent is added on the top of the column and solvent allowed to run down. 13

14. Elution development procedure of two solute drug A and B, here A is more strongly adsorbed than B , and E is the eluent . B is less absorbing power than A. 14

15. In this principle, the mixture to be separated is dissolved in a suitable solvent and allowed to pass through a tube containing adsorbent. The components which has greater absorbing power is adsorbed in the upper part of the column. The next component is adsorbed in the lower portion of the column which has less adsorbing power than the first components. The process is continued. As a result the materials are partially separated and adsorbed in the various parts of the column. The type of interaction between the stationary phase and the solute is reversible in nature. 15

16. Instrumentation techniques of column chromatography Column characteristics Adsorbents Preparation of the adsorption column Packing of the columns Mobile phase used in cc Introduction of sample Separation of compounds Detection of the compounds Recovery of components 16

17. Instrumentation techniques of column chromatography 17

18. (A) Column characteristics The materials of the column is mostly good quality neutral glass since it should not be affected by solvents ,acids or alkalis. It is obtained commercially with spring loaded stopcocks with a sintered glass disc at the bottom. An ordinary burette can also be used as column for separation The length of the columns depends upon Affinity of compounds towards the adsorbent used. Number of the compound to be separated . Type of adsorbent used . Quantity of the sample. A stopcock fitted to a column is allow to control the flow rate of solvent and a sintered glass plate or small plug of glass wool is used at the bottom to support the adsorbent used in columns.

19. (b)Adsorbents Based upon their adsorbent activity and good filtration property they can be classified into three parts :- Weak adsorbents Intermediate adsorbents Strong adsorbents These adsorbents are sold in different mesh sizes, as indicated by a number on the bottle label such as silica gel 60 or silica gel 230-400. The larger the mesh size, the smaller the adsorbent particles. Silica gel 70–230 is used in gravity column chromatography Silica gel 230–400 is used in flash chromatography. The most commonly used adsorbents for column chromatography are Silica gel (SiO 2 ) and Alumina (Al 2 O 3 ).

20. (C ) PREPARATION OF THE ADSORPTION COLUMN At first a plug of cotton wool or glass wool is placed in the bottom of the column and pressed down evenly. Then some cleaned, washed sand can be poured on the top of the plug to form a thin layer. The sand serves to give a flat base to the column of adsorbent when cotton wool is used instead of sinter glass. 20

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22. (d) Packing of the columns The adsorbent is applied to the column in two ways: 1. Dry packing: In this method the dry adsorbent is poured to the column directly . Vibration/vacuum pump is the applied to get rid of air bubbles then the mobile phase is passed through the adsorbent. Demerits: Air bubbles are entrapped between mobile phase and stationary phase Column may not be uniformly packed Cracks appear in the adsorbent present in the column. 22

23. 2. Slurry packing (Wet method): This is the ideal technique. Slurry is prepared by taking at first small amount silica (10-20 gm) & least polar solvent (PE) & mixed well in a beaker & then poured into the column. This process is continued for several times until the stationary phase is firmly packed in the column. Sand may be added after the slurry. 23

24. PRECAUTION: After setting the adsorbent the excess solvent is allowed to drain out by the outlet duct but a “ head” or layer of solvent should always cover the adsorbent. Otherwise cracks will develop in the column and becomes useless for chromatography because the solvent runs through the cracks rather than between the particles of adsorbent. 24

25. (E) Mobile phase used in CC Different solvents are used for this purpose such as: 1. Petroleum ether 2. Carbon tetra chloride 3. Chloroform 4. Acetone 5. Benzene 7. Toluene 8. Water 9. Pyridine etc. Choice of an eluting solvent is very important for the successful separation of a mixture of chemical compounds. Polar solvents elute polar molecules more rapidly and vice-versa. 25

26. (f) Introduction of sample The sample is placed at the top of the column (stationary phase) as a band either by two ways; i.e. Wet application and Dry loading 1. Wet application: Dissolve the sample in the initial mobile phase and apply by pipette to the top of the column. This is very good method but in most of cases the samples are not soluble in the initial mobile phase. 2. Dry loading: Dissolve sample in any volatile solvent. The sample solution is then adsorbed on small weight of adsorbent (stationary phase) and the solvent is allowed to evaporate. The dry adsorbent loaded with the sample is then applied to the column. 26

27. Eluting the sample: Once the sample solution is added onto the silica, eluent is added to the top of the column at a rate sufficient to ensure a “Head” of liquid on the top layer of sand at any point of chromatogram development. The composition of the eluent can be changed as the column progresses. The constituents of the sample will pass down the column at varying rates. 27

28. ( g) separation of compounds (a) isocratic elution (same) (b) Gradient elution ( gradually ) Isocratic elution technique: Iso means same/similar. In this elution technique, the same solvent or solvent system of same polarity is used throughout the process of separation. E.g.: chloroform, petroleum ether etc. Gradient/Stepwise elution technique: The solvents of gradually increasing polarity or increasing elution strength are used during the process of separation. Initially low polar solvent is used followed by gradually increasing the polarity. E.g.: Initially benzene then chloroform, ethyl acetate, methanol etc. 28

29. (H) RECOVERY OF COMPONENTS: The eluate is collected as it is released from the column in small volumes of 5, 10 or 15ml test tubes. Analyzing the fractions: Then the fractions are analyzed by thin-layer chromatography. Similar fractions are combined. If the fraction contains a single compound the solvent is evaporated to obtain the pure compound. But if the fraction contains more than one component they are separated using another suitable technique. 29

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31. (I) Detection of the compounds By visual examination of the column: After developing the chromatogram by running the solvent, different bands of components are formed. If the bands are colored then we can detect the component visually if color of the component is known. Eluting the various components with solvents: It is however more convenient to complete the chromatogram by eluting the various components with solvents. The eluate is collected as a large number of fractions, each of small volume. The large number of fractions assists in obtaining better separation of components. Each fraction is examined appropriately for the presence of compounds by: By simple spot tests by paper or TLC By passing UV light compounds can be detected visually. By addition of reagents 31

32. Other methods of visualization: Measuring changes in pH, Measuring changes in conductivity, Measuring changes in refractive index, Measuring absorption of UV light, Measuring fluorescence (for quinine). Adding fluorescein (0.04%) to the adsorbent and then viewing the column under UV light. The background will fluoresce except in those areas where chemicals are present and they will appear as dark bands. Adding dimethyl amino azobenzene with silica gel to identify alkaloids. The silica will appear red colored except where the alkaloids are present. Adding ethereal solution of ferric chloride with alumina to identify organic compounds which react with ferric chloride to give colored compounds. 32

33. Factors affecting separations Factors due to stationary phase: Particle size of the stationary phase: Reducing the particle size increases the surface area and improves separation. However, reduction of the particle size will decrease the flow rate of the mobile phase. In HPLC we use very fine particles to get very good separation. The flow rate problem is solved by the use high pressure pumps to push the mobile phase through the stationary phase. Columns are made of stainless steel to withstand the high pressure. Adsorbent activity: The choice of the suitable adsorbent is very important. Uniformity of packing of the column: If the stationary phase is not packed uniformly then the bands will be irregular and resulting in poor separation. Concentration of the mixture: the proper ratio between sample to be separated and the amount of stationary phase is very important. Too much samples resulted in poor separation. 33

34. Factors due to mobile phase: Selection of the proper mobile phase: Very polar mobile phase will wash out all components without any separation. On the other hand very non polar mobile phase will result in broad band and poor separation. Rate of flow: Slower flow rate usually resulted in a better separation and narrower bands. Consistency of flow: The continuous flow of the mobile phase during the whole experiment gives better separation. 34

35. Factors due to column: Column dimensions: Increasing the length of the column improve separation. However, that usually leads to slower flow rate. Column temperature: Increasing the temperature usually reduces the adsorption power of the stationary phase and increase elution speed. This may leads to decrease in the separation efficiency. 35

36. Column partition chromatography The separation of a component in partition chromatography is based on the partition co-efficient of the individual component of a mixture between a liquid stationary phase and gaseous or liquid mobile phase. But in case of column partition chromatography, the mobile phase is a liquid. 36

37. PC Process 37

38. Partition chromatography may be conducted in either of two ways: Parameter Normal phase Reversed phase Stationary phase Polar Non-polar Mobile phase Non-polar Polar Compound eluted first Non-polar Polar Compound eluted last Polar Non-polar Example of stationary phase Silica gel C18, C8-bonded phase Frequently used in CC, TLC HPLC 38

39. Stationary phase In partition column chromatography, solid materials are used solely as a thin layer to support the liquid stationary phase. Usually the liquids used as stationary phase is polar in nature. For example, water or aqueous buffer solution may be used as stationary phase. Silica gel is the most convenient supporting material for the liquid stationary phase because it is capable of retaining considerable volume of liquid phase. Cellulose powder can also be used as supporting material. Organic liquids or solvents may also be used as stationary phase. In this case, kieselguhr is commonly used as supporting material. 39

40. Mobile phase The solvent system which is used for column adsorption chromatography may also be used for partition column chromatography. Reversed-phase C18 columns (ODS): octadecyl carbon chain (C18) is bonded with. Because of steric effects, not all of the hydroxyl groups of the silica are derivatized by the ODS reagent, so the remainder are reacted with trimethylsilane in a process called capping to reduce adsorption effects. 40

41. Advantages: CC is advantageous over most other chromatographic techniques because it can be used to separate and purify substantial quantities of components from a mixture. Disadvantages: 1. Packing of the column requires some technical skill. 2. The technique is time-consuming and tedious, especially for larger samples utilizing gravity column chromatographic technique. 3. Requires large amount of solvents. 4. It requires constant attention while the experiment is being performed . 5. Collection vessels must be frequently changed and solvent should be added continuously to the top of the column at a rate sufficient to cover the adsorbent. 41

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