Column Chromatography technique presentation.pptx

Column Chromatography technique presentation

1. COLUMN CHROMATOGRAPHY Presented By- Dip Roy BSc Student Department of Chemistry University of Barishal,Bangladesh

2. Column chromatography was devoloped by the American chemist D.T Day in 1900 .M.S. Tswett , the Polish botanist, in 1906 used adsorption columns in his investigations of plant pigments.

3. COLUMN CHROMATOGRAPHY Column chromatography is a chromatography method used to isolate a single chemical compound from a mixture. In order to actually perform the separation, a glass column is assembled (as shown in the picture). A glass column was taken with a stopcock attached at the bottom, inserted a cotton plug at the bottom of the column and packed the column with a slurry of silica gel (prepared in an organic solvent).

4. COLUMN CHROMATOGRAPHY Once the column was packed, poured the reaction mixture over the bed of silica from the top of the column carefully, with the aid of a glass pipette. Then the stopcock was opened and let the solvent run slowly through the column and constantly kept adding solvent from the top of the glass column.

5. COLUMN CHROMATOGRAPHY The reaction mixture started separating into three distinct bands respectively. The individual bands was collected in separate flasks and was thus able to obtain desired product. The different components of the analyte exhibit varying degrees of adhesion to the silica, and as a result they travel at different speeds through the stationary phase as the solvent flows through it, indicated by the separation of the different bands.

6. COLUMN CHROMATOGRAPHY Higher the adsorption to the stationary phase, the slower the molecule will move through the column. Higher the solubility in the mobile phase, the faster the molecule will move through the column.

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