Comparison between Replication of Prokaryotes and Eukaryotes

Comparison between Replication of Prokaryotes and Eukaryotes
Mujahid Hussain
(M.Phil Botany)
Department of Botany
University of Sargodha, Sargodha ,Punjab, Pakistan

1. Comparison between Replication of Prokaryotes and Eukaryotes Mujahid Hussain ( M.Phil Botany) Department of Botany University of Sargodha, Sargodha ,Punjab , Pakistan 8/2/2017 Mujahid Hussain(M.Phil Botany) Department of Botany, UOS, [email protected] 1

2. Comparison between Replication of Prokaryotes and Eukaryotes DNA Replication in Prokaryotes In prokaryotes replication start from ori c site known as Origin of replication site.   DNA Replication in Eukaryotes The genome of Eukaryotes is so much large so its speed is very slow that is 50 nucleotide per second. And it has many points from where replication is started. It has many origin of replication. 8/2/2017 Mujahid Hussain(M.Phil Botany) Department of Botany, UOS, [email protected] 2

3. It has small genome so therefore . After each 30,000 or 300,000 nucleotides, there is a new origin of replication site. 8/2/2017 Mujahid Hussain(M.Phil Botany) Department of Botany, UOS, [email protected] 3

4. Ori c site. Those sequences from where replication starts is called ARS site Autonomous Replication Site 8/2/2017 Mujahid Hussain(M.Phil Botany) Department of Botany, UOS, [email protected] 4

5. . Here DNA-A protein attach with ori c site, which is single protein. Here at ARS, attach a multiple complexes of group of protein called origin recognition complexes ORC: it is a multiprotien complexes. 8/2/2017 Mujahid Hussain(M.Phil Botany) Department of Botany, UOS, [email protected] 5

6. Here DNA-C protein carry the the DNA-B protein. Here a multiprotiens do this work. These are monochromosomes maintenance protein ( MCM 6 , mco ) These act as DNA-B protein and cell division cycle6 or ( CDC 6 ). CDT act as DNA-c protein there are 2 multiprotien complexes which act as DNA-C protein. 8/2/2017 Mujahid Hussain(M.Phil Botany) Department of Botany, UOS, [email protected] 6

7. when DNA-C protein has done its work, topoisomerase come to attach on both sides. Same is the case with, topoisomerase 1 or toporoisomerase2 attach on both sides. 8/2/2017 Mujahid Hussain(M.Phil Botany) Department of Botany, UOS, [email protected] 7

8. Here SSBP attach with the strand of DNA and keep them in unwind protein, so that they can’t rewind again. . RNA premise attach the primer. Replication proteins a “RPA” which is a multiprotien complex. It stops to rewind of nucleotides of eukukarytic DNA. 8/2/2017 Mujahid Hussain(M.Phil Botany) Department of Botany, UOS, [email protected] 8

9. Here DNA polymerase1. DNA polymerase2. DNA polymerase3. Involve in replication Here DNA polymerase alpha DNA polymerase delta and DNA polymerase epsilon. Involve in replication 8/2/2017 Mujahid Hussain(M.Phil Botany) Department of Botany, UOS, [email protected] 9

10.   All RNA primase makes the primer. DNA polymerase alpha makes the primer. 8/2/2017 Mujahid Hussain(M.Phil Botany) Department of Botany, UOS, [email protected] 10

11. DNA polimarse3 is involved in main replication process. DNA polymers Delta carry out the main replication. 8/2/2017 Mujahid Hussain(M.Phil Botany) Department of Botany, UOS, [email protected] 11

12. Here DNA polimarse1 or DNA polimarse2 carry out the proof reading. DNA polymerase epsilon do the poof reading. 8/2/2017 Mujahid Hussain(M.Phil Botany) Department of Botany, UOS, [email protected] 12

13. Speed is 1000 nucleotide per second. Replication speed is 50 nucleotide per second 8/2/2017 Mujahid Hussain(M.Phil Botany) Department of Botany, UOS, [email protected] 13

14. Terminating proteins are involved or Tus-Ter complexes are present at the end of strands. 17. Replication is bidirectional. Here long adenine chains ( of 100-200 nucleotide) called talomess at the end of strands. 15. Replication is also bidirectional. 8/2/2017 Mujahid Hussain(M.Phil Botany) Department of Botany, UOS, [email protected] 14

15. Replication is bidirectional. Replication is also bidirectional. 8/2/2017 Mujahid Hussain(M.Phil Botany) Department of Botany, UOS, [email protected] 15