Components of blood (For Transfusion)

Components Of Blood (For Transfusion)
• Each unit of blood is tested for evidence of hepatitis-b,
hepatitis-c, Human Immune deficiency Virus I & II.
• The blood is then processed into sub-components.
• Whole blood
• Packed cell volume
• Fresh frozen plasma
• Platelets
• Cryoprecipitate

1. Components Of
Blood (For Transfusion)
Mr. Harshad Khade
MSc. Medical Technology (OTA)
Symbiosis International university, Pune.

3. Components Of Blood (For Transfusion)
•Each unit of blood is tested for evidence of hepatitis-b,
hepatitis-c, Human Immune deficiency Virus I & II.
•The blood is then processed into sub-components.
•Whole blood
•Packed cell volume
•Fresh frozen plasma
•Platelets
•Cryoprecipitate

5. Whole Blood
•Whole blood is unseparated blood containing an
anticoagulant –preservative solution.
•One unit of whole blood contains-
•450 ml of donor blood.
•50 ml of anticoagulant-preservative solution.
•Haemoglobin approx.12g/ml & haematocrit 35%-45%.
•No functional platelets.

6. •Stored between +2 and +6 degrees centigrade in a blood
bank refrigerator.
•Transfusion should be started within 30 minutes of removal
from the refrigerator and completed within 4 hours of
commencement because changes in the composition may
occur due to red cell metabolism.

8. Packed Red Cells
•Packed red cells are cells that are spun down and
concentrated.
•One unit of packed red cells is approx. 330 ml and has a
haematocrit of 50-70%.
•They are stored in a SAG-M (saline-adenine-glucose-
mannitol) solution to increase their shelf life to 5weeks at +2
to +6degrees centigrade.
•1 unit of PRBCs = raises haematocrit by 2-3%

10. Fresh Frozen Plasma
•Plasma is the liquid component of blood; it has proteins called clotting
factors
•Fresh frozen plasma is rich in coagulation factors.
•Expands blood volume and provides clotting factors.
•It is separated from whole blood and stored at-40 to -50 degrees
centigrade with a 2year shelf-life.
•Contains no RBCs .
•1 unit of FFP = increases level of any clotting factor by 2-3%.
•It is the first line therapy in the treatment of coagulopathic
haemorrhage.

12. Platelets
•Also known as thrombocytes
•Tiny cell structures necessary in blood clotting process
•Replaces platelets in clients with bleeding disorders, or
platelet deficiency
•1 unit = increases the average adult client’s platelet count by
about 5,000 platelets/microliter

13. •Autologous Red Blood Cells
•Used for blood replacement following planned elective
surgery
•Must be donated 4-5 weeks prior to surgery.
•Albumin and Plasma Protein Fraction
•Blood volume expander
•Provides plasma protein

15. Cryoprecipitate
•Cryoprecipitate is a supernatant precipitate of fresh frozen
plasma and is rich in factor VIII and fibrinogen.
•It is stored at -30 degrees centigrade with a 2 years shelf life.
•Indicated in low fibrinogen states (<1g/l) or in cases of factor VIII
deficiency (haemophilia-a), von will brand's disease and as a
source of fibrinogen in disseminated intravascular coagulation.
•Pooled units containing 3-6 gm fibrinogen in 200-500 ml raises
the fibrinogen level by approx. 1g/L.
•Must be infused within 6 hours.

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Thank You