CPCSEA Guidelines/Animal handling/Guidelines for laboratory animal/experiments on animal.pptx

In this ppt you will get to know about the Anesthesia process to animal, detail about the Euthanasia, Physical facilities for the lab animal, Environment, Animal husbandry, Record keeping, standard operating procedure, Personnel and Training of the person working under animal lab, Transport of animals

1. CPCSEA GUIDELINES Presented by: Md Inzamamul haque Department of Pharmaceutical sciences HNB Garhwal University Uttarakhand (A Central University) Presented to: Dr.Abdul Farukh

2. TOPICS 1 1.Anesthesia 2.Euthanasia 3.Physical facilities 4.Environment 5.Animal husbandry 6.Record keeping 7.SOPs 8.Personnel and training 9.Transport of animals

3. 2 CPCSEA HNBGU Anesthesia is a medical treatment/process that prevents organism from feeling pain during surgery. It must also be ensured that the anesthesia is given for the full duration of experiment and at no stage the animal is conscious to perceive pain during the procedure. Neuromuscular blocking agents must not be used without adequate general anesthesia. ANESTHESIA

4. 3 CPCSEA HNBGU

5. EUTHANASIA Euthanasia should be resorted to events where an animal is required to be sacrificed to reduce suffering or to limit spread of infections or for termination of an experiment or for other ethical reasons . 4 CPCSEA HNBGU

6. The method should in all cases meet the following requirements: (a) Death, without causing anxiety, pain or distress with minimum time lag phase. (b) Minimum physiological and psychological disturbances. (c) Compatibility with the purpose of study and minimum emotional effect on the operator. (d) Location should be separate from animal rooms and free from environmental contaminants. • Tranquilizers have to be administered to larger species such as monkeys, dogs and cats before a procedure of euthanasia. e.g.(diazepam, alprazolam, serotonin, equanil ) 5 CPCSEA HNBGU

7. PHYSICAL FACILITIES Building materials- Moisture-proof, fire-resistant, seamless materials are most desirable for interior surfaces including vermin and pest resistance. Corridor- Wide enough to facilitate the movement of personnel as well as equipment and should be kept clean. Utilities- Water lines, drain pipes and electrical connection. Animal room doors- Rust, vermin and dust proof. It is properly within their frames and provided with an observation window. Floors- Smooth, moisture proof, non-absorbent, skid-proof. Drains- Floor drains are not essential in all rooms used exclusively for housing rodents. 6 CPCSEA HNBGU

8. Walls & ceilings- Free of cracks, unsealed utility penetrations, or imperfect junction with doors, ceilings, floors and corners. Storage areas Separate storage areas should be designed for feed, bedding, cages and materials not in use. Facilities for sanitizing equipment and supplies- An area for sanitizing cages and ancillary equipment is essential with adequate water supply. Experimental area- Separate area from the place where animals are housed. 7 CPCSEA HNBGU

9. ENVIRONMENT Temperature and humidity control :- • Prevents variations due to changing climatic conditions or differences in the number and kind of room occupants. • Air conditioning is effective in regulating environmental parameters. • Temperature range- 18 to 29°C (64.4 to 84.2°F) Relative humidity range- 30% to 70% Ventilation :- The animal facility and human occupancy areas should be ventilated separately. 8 CPCSEA HNBGU

10. Power and lighting :-The electrical system should be safe and provide appropriate lighting and a sufficient number of power outlets. Lighting system must be installed that provides adequate illumination while people are working in the animal rooms and a lowered intensity of light for the animals, for e.g. fluorescent lights. A time-controlled lighting system should be used to ensure a regular diurnal lighting cycle wherever required. Noise control:- Noise free environment. 9 CPCSEA HNBGU

11. ANIMAL HUSBANDRY Caging & housing system :- • Designed carefully to facilitate animal well being, meet research requirements, and minimize experimental variables. • Adequate ventilation. • Meet the biological need of animal. • Keep the animal dry and clean. • Cages made of steel or painted steel (Annexure - 3). • Feeding and watering devices should be easily accessible for filing, changing, cleaning and servicing. • Provide space that is adequate, permit freedom of movement and normal postural adjustments, and have a resting place appropriate to the species (Annexure - 3). 1O CPCSEA HNBGU

12. Provide a comfortable environment. Provide an escape proof enclosure that confines animal safety provide easy access to food and water. • Facilitate research while maintaining good health of the animals. • Constructed of sturdy, durable materials and designed to minimize cross-infection between adjoining units. Cages should have smooth, impervious surfaces that neither attract nor retain dirt and a minimum number of ledges, angles, and corners. • Cages, runs and pens must be kept in good condition to prevent injuries to animals, promote physical comfort, and facilitate sanitation and servicing . 11 CPCSEA HNBGU

13. Sheltered or outdoor housing :- Shelter should be accessible to all animals, have sufficient ventilation, and be designed to prevent build up of waste materials and excessive moisture. Houses, dens, boxes, shelves, perches, and other furnishings should be constructed in a manner and made of materials that allow cleaning or replacement. Ground-level surfaces of outdoor housing facilities can be covered with absorbent bedding, sand, gravel, grass, or similar material that can be removed or replaced when needed to ensure appropriate sanitation. • Buildup of animal waste and stagnant water should be avoided. 12 CPCSEA HNBGU

14. Social environment :- The effects of social environment on caged animals vary with the species and experience of the animals. Population density can affect reproduction, metabolism, immune responses, and behavior . 13 CPCSEA HNBGU

15. RECORD KEEPING The Animal House should maintain following records : 1. Animal House plans, which includes typical floor plan, all fixtures etc. 2. Animal House staff record - both technical and non- technical. 3. Health record of animals. 4. All SOPs relevant to the animals. 5. Breeding, stock, purchase and sales records. 6. Minutes of institute Animals Ethics Committee Meetings. 7. Records of experiments conducted with the number of animals used (copy of Form b). 8. Death Record. 9. Clinical record of sick animals. 10. Training record of staff involved . 14 CPCSEA HNBGU

16. STANDARD OPERATING PROCEDURE Maintain standard operating procedure (SOP) describing procedures/ methods adapted with regard to animal husbandry, maintenance, breeding, animal house microbial analysis and experimentation record. SOP should contain following items :- • Name of author • Title of SOP • Date of preparation • Reference of previous SOP on the same subject and date • Location and distribution of SOPs with sign of each recipient • Objectives • Detailed information of the instruments used in relation with animals • Normal value of all parameters. 15 CPCSEA HNBGU

17. PERSONNEL AND TRAINING The selection of animal facility staff, particularly the staff working in animal rooms or involved in transportation, is a critical component in the management of an animal facility. The staff must be provided with all required protective clothing (masks, aprons, gloves and gumboots and other footwear) while working in animal rooms. Facilities should be provided for change over with lockers, wash basin, toilets and bathrooms to maintain personal hygiene. Regular medical check-up is arranged for the workers. Persons working in animal house do not eat, drink, smoke in animal room and have all required vaccination. Initial in-house training of staff at all levels is essential. 16 CPCSEA HNBGU

18. TRANSPORT OF LAB ANIMALS The main considerations for transport of animals are • The mode of transport, • The containers, • The animal density in cages, • Food and water during transit, • Protection from transit infection, injuries and stress . 17 CPCSEA HNBGU

19. Summary The CPCSEA (Committee for the Purpose of Control and Supervision of Experiments on Animals) is a statutory body under India’s Ministry of Environment, Forest and Climate Change. It regulates and oversees the ethical use of animals in scientific research and experiments, ensuring compliance with humane practices under the Prevention of Cruelty to Animals Act, 1960 . CPCSEA registers institutions, reviews research proposals, and enforces guidelines to minimize animal suffering. It requires establishments to form Institutional Animal Ethics Committees (IAECs) to monitor compliance and promotes alternatives to animal testing. By balancing scientific advancement with animal welfare, CPCSEA aligns India with global ethical standards. 18 CPCSEA HNBGU

20. Thank you Md Inzamamul haque [email protected]