CPCSEA Guidelines for Laboratory Animal facility.pptx

Need of CPCSEA :-
To enhance animal well being and quality.
To improve laboratory animal facility.
To enhance biological knowledge.
To ensure that lab animals are well maintained

1. CPCSEA Guidelines for Laboratory Animal facility:- Anaesthesia , Euthanasia & Physical facilities SMBT College of Pharmacy, Dhamangoan , Nashik Dr. Manoj Ramesh Kumbhare Professor

2. Content :- Introduction Anaesthesia Euthanasia Physical facility

3. CPCSEA :- means committee for the purpose of control and supervision of experiments on animals. Need of CPCSEA :- To enhance animal well being and quality. To improve laboratory animal facility. To enhance biological knowledge. To ensure that lab animals are well maintained.

4. Anaesthesia Defination :- drug which cause loss of sensation. Types:- 1. General anaesthesia 2. Regional /Nerve block anaesthesia 3.Local anaesthesia

5. 1) General Anaesthesia :- Loss of sensory perception of entire body. unconscious person. Route – IV infusion/inhalation Eg :- Benzodiazepines( midazolin /diazepam)

6. 2) Regional / Nerve block Anaesthesia :- Loss of sensation over specific region of body(arm, leg,) No unconscious. Person remain conscious but can not feel any pain any part of body. Method of administration- spinal block, Epidural block, Regional block Eg :- Propofol , Ketamine .

7. 3 ) Local Anaesthesia :- Loss of sensation over a small limited area of body. No unconsciousness. Person remains conscious particular part of body get loss of sensation. Method of administration- Topical application Eg :- Lidocaine , Lignocaine Hydrochloride Injection IP solution.

8. Euthanasia It is resorted to events where an animal is required to be sacrificed on termination of an experiment or otherwise for ethical reasons. Procedure carried out quickly and painlessly in an atmosphere free from fear or anxiety. The choice of method will depend on the nature of study, the species of animal to be killed. Methods :- Physical methods- Electrocution, exsanguination, decapitation , cervical dislocation. Drug administration- Overdose of chloral hydrate, Ketamine, Chloroform.

9. Requirements of Method :- Death, without causing anxiety, pain or distress with minimum time lag phase. Minimum physiological and psychological disturbances. Compatibility with the purpose of study and minimum emotional effect on the operator. Location should be separate from animal and free from environment contaminants .

10. Physical facilities:- Building material:- should be moisture proof, fire resistant, seamless (smooth) materials most desirable for interior surfaces including vermin and pest resistance. Corridor :- wide enough to facilitate the movement of personnel as well as equipment and should be kept clean. Utilities :- water lines, drain pipes & electrical connections should be outside the animal rooms Animal room door :- not be rust, dust proof ,vermin. fit properly in their frames. door closures may also be provided. Exterior windows :- not recommended for small animals ,only for large animals.

11. Floors :- monolithic / epoxy smooth, moisture proof, nonabsorbent, skin-proof, resistant to wear, acid, solvents, Drains :- floor drains are not essential in all rooms, drainage must be adequate to allow rapid removal of water , to prevent the high humidity, drainage must be adequate to allow rapid removal of water and drying surfaces.at the inlet and outlet of the drains should be fitted with wire mesh guard to prevent wild rodent entry Walls & Ceilings :- walls should be free of cracks, imperfect junctions with doors, ceilings, floors, and corners. Storage areas :- separate storage areas should be designed for feed , bedding, cages and materials not in use. Refrigerator storage, separated from other cold storage , is essential for storage of dead animals and animal tissue waste.

12. Reference :- Jairam ramesh ; CPCSEA guidelines for laboratory animal Facility;7 th january 2010; page no.22-56. CPCSEA guidelines, Govt. of I ndia. slideshare Indian journal of Pharmacology, 2003, page no.257-274.

13. Thank You.