CRYOPRESERVATION_Techniques Jan2021.pptx

cryopreservation techniques in animal cell culture

1. CRYOPRESERVATION

2. cryopreservation The ability to freeze and thaw with retention of viability Provides flexibility in human infertility therapy when gametes or embryos are handled  in vitro  because frozen tissue can be stored indefinitely in liquid nitrogen at -196°C Boon to animal husbandry – art Gamete / embryo banking

3. Principle of cryopreservation Intracellular ice crystal formation Exposure to high solute conc. / Osmotic shock in freeze / thawing Osmotic challenge in the temperature transition depends on cell's permeability coefficient, its surface area, and the osmotic gradient between outside and inside Cellular dehydration & lowering of freezing point

4. Cyrosurvival depends on cell type , cryoprotectant selection, cryoprotectant addition, seeding , cooling rate, final cooling temperature, hydration state of the cells, warming rate, and cryoprotectant removal