Culture characteristics of bacteria

There are different bacterial characteristics . These include cultural characteristics, cellular characteristics, and biochemical characteristics. The simplest of these to observe are the cultural characteristics. Learning the cultural characteristics of the organisms we use in the lab will help you greatly, especially when working on your morphological unknown.

1. Culture characteristic of bacteria Archana Soni Assistant Professor Department of Biotechnology, SSMV, Bhilai

2. Bacteria grow on solid media as colonies . A colony is defined as a visible mass of microorganisms all originating from a single mother cell .

3. Colony Morphology These characters used to accurately describe the morphology of a bacterial colony: - Size -Shape -Color (also known as pigmentation) -Texture -Height (elevation) -Edge (margin) Key features of bacterial colonies are important criteria for their identification.

4. COLONY CHARACTER ON AGAR PLATE

5. Colony shape on agar plates

6. Rhizoid Circular Pinpoint

7. Margin of bacterial colony

8. Undulate filamentous

9. Elevation of bacterial colony

10. Raised margin Umbonate

11. Size of the bacterial colony Small Medium large

12. Texture of bacterial colony Dry Moist Viscid (stick to loop) Mucoid (mucus-like)

13. Colour of the colonies (pigmentation) Some bacteria produce pigment when they grow in the medium.

14. Opacity of the bacterial colony Opaque (not clear) Translucent (clear) Iridescent(shine)

15. COLONY CHARACTER ON AGAR SLANT

16. Growth on agar slants

17. beaded Filiform

18. GROWTH IN NUTRIENT BROTH

19. Growth in nutrient broth

20. LAB EXERCISE -Student will identify the growth patterns on the nutrient agar plates and slants, and in the nutrient broth tubes.