Cyclic amp

SHORT PRESENTATION

1. Vydehi veeramalla Final yr pg Department of Biochemistry

2. CONTENTS CELL SIGNALLING TYPES OF SECOND MESSENGERS CHARECTERISTICS OF SECOND MESSENGERS HORMONES ACTING THROUGH CYCLIC AMP MECHANISM OF ACTION OF CYCLIC AMP EFFECTS OF CYCLIC AMP

3. HISTORY Earl Wilbur Sutherland Jr. – 1971 Nobel Prize in Physiology or Medicine Epinephrine – liver - glycogen to glucose – cAMP

4. INTRODUCTION Cyclic AMP is a cyclic nucleotide Chemically it is a 3’5’ adenosine monophosphate It is synthesized in tissues from ATP under influence of ADENYLYL CYCLASE in presence of Mg ion Degraded to 5’AMP in by P hosphodiesterase It is a Second M essenger

5. cell signaling- 3 processes A series of signaling events that mediate the response to each stimulus. specific amplified regulated coordinated. receptors plasma membrane , cytoplasm or nucleus.

6. Second Messengers cAMP pathway cGMP pathway IP 3 / DAG pathway Calcium as a second messenger Eicosanoids

7. Second Messengers General characteristics Low amounts in resting state Regulated synthesis Regulated destruction Act through other proteins

8. hCG LH FSH LPH PTH CALCITONIN Epinephrine ( β 1 , β 2 ) Norepinephrine Glucagon CRH ACTH MSH  TSH Somatostatin Acetylcholine M 2 Angiotensin II Epinephrine α 2 ATIVATE ADENYLYL CYCLASE INHIBIT ADENYLYL CYCLASE AVP/ADH

9. G Protein-Linked Receptors

10. G-PROTEIN COUPLED RECEPTOR

11. tes Activation of G Proteins AC ADENYLYL CYCLASE

12. G PROTEIN ACTIVATES ADENYLYL CYCLASE

13. Cyclic AMP

14. PROTEIN KINASE A - ACTIVATION

15. EFFECTS OF CYCLIC AMP ALTERED GLYCOGEN METABOLISM BY ENZYME REGULATION ALTERED GENE TRANSCRIPTION HORMONE ESTRADIOL SECRETION ALTERED ION TRANSPORT - HCL SECRETION IN STOMACH WATER REABSORPTION BY ANTI DIURETIC HORMONE CAFFEINE – PHOSPHODIESTERASE INHIBITORS DRUGS – SALBUTAMOL , THEOPHYLLINE

16. ENZYME REGULATION GSD VIII,IX GSD X

17. GENE TRANSCRIPTION TRANSCRIPTION FACTOR COACTIVATOR

18. ESTRADIOL SECRETION

19. STOMACH - HCL SECRETION HISTAMINE

20. KIDNEY - WATER REABSORPTION ANTI DIURETIC HORMONE

21. PHOSPHODIESTERASE INHIBITORS

22. EPINEPHRINE NOREPINEPHRINE SALBUTAMOL DRUGS THEOPHYLLINE PDE

23. Summary Cyclic amp is a mediator of hormone action Second messenger Activates protein kinase A Causes glycogenolysis Causes lipolysis Modulates Transcription Activates Hormone secretion Increases gastric secretion Regulates cell permeability to water