Detailed Project Report on PROTEIN HYDROLYSATE FROM SHRIMP/FISH WASTE

Protein hydrolysates have attracted increasing interest as potential ingredients for many health-promoting functional foods due to biologically active peptides. The recovery of proteins or peptides from shrimp waste by hydrolysis has been widely investigated. Endogenous proteases were reported in hepatopancrease.

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ENGINEERS INDIA RESEARCH INSTITUTE
4/54, Roop Nagar, Delhi-110007 (India)
Phone: [phone]
E-mail: [email protected]
Website: www.eiriindia.org



PROTEIN HYDROLYSATE FROM
SHRIMP/FISH WASTE
[EIRI/EDPR/4687] J.C.: 2904XL


Protein hydrolysates have attracted increasing interest as potential ingredients for
many health-promoting functional foods due to biologically active peptides. The
recovery of proteins or peptides from shrimp waste by hydrolysis has been widely
investigated. Endogenous proteases were reported in hepatopancrease.

Under optimal condition, those proteases could autolysis. Autolysis and enzymatic
hydrolysis of cephalothorax depend on several factors including pH, temperature,
time as well as enzyme/substrate ratio reported that optimum autolysis condition
for protein recovery of cephalothorax from Penaens vannamei included 50ºC, pH
7.85 and a substrate concentration at 23% (w/v), in which the highest degree of
hydrolysis (45%) was obtained. The autolysis of shrimp head by gradual increase
in temperature (40- 60ºC) at 5ºC/30 min resulted in the higher protein recovery
(87.4%), compared with autolysis performed at different single temperature (40,
50, and 60ºC) (43.6- 73.6%). Additionally, a number of commercial proteases have
been used for the production of protein hydrolysis from seafood processing by-
products. Protein recovery of hydrolysate from shrimp waste using Alcalase was
higher than those using Neutrase, Protamex and Flavourzyme. The protein
hydrolysate from shrimp waste of deep-water pink shrimp was produced by
Alcalase hydrolysis.

Hydrolysate contained 80.8% protein, 2.74% lipid, 14.4% ash, 1.13% chitin and
1.08 µg carotenoid/g sample.

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To maximize the hydrolysis of proteins from cephalothorax, autolysis could be
exploited. The liberated protein or peptides more likely served as the
proteinaceous substrate for subsequent hydrolysis by commercial proteases. The
hydrolysates containing varying peptides from different hydrolysis processes might
possess different compositions as well as bioactivities, especially antioxidant
activity. Nevertheless, a little information regarding the prior autolysis in
combination with subsequent enzymatic hydrolysis for production of protein
hydrolysate from shrimp cephalothorax exists.

Protein Hydrolysate is a solution of protein hydrolyzed into canstituent amino
acids.

Amino acids are the main components of protein, protein are found in all living
organism and play an important role in living cells. Approximately 20 amino acids
are the common constituents of protein.

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CONTENTS


INTRODUCTION 7
USES AND APPLICATIONS 11
PROPERTIES & CHARACT ERISTICS 12
PROTEIN ISOLATES 12
PK & PI VALUES AT 25OC AND SOLUBILITY TO AMINO ACIDS 12
CHEMICAL PROPERTIES:- 13
STRECKER SYNTHESIS:- 13
BUCHERER SYNTHESIS:- 13
A-AMINO ACIDS FORM COPPER SALTS: - 14
B.I.S. SPECIFICATION 15
PROTEIN HYDROLYSATE MARKET ANALYSIS 17
RISE IN POPULARITY OF VEGAN DIET 18
ASIA-PACIFIC DRIVES PROTEIN HYDROLYSATE MARKE T 19
PROTEIN HYDROLYSATE MARKET LEADERS 20
GLOBAL MARKET PROSPECT OF PROTEIN HYDROLYSATE 21
PROTEIN HYDROLYSATE 24
PRODUCTION OF HYDROLYSATE FROM SHRIMP 25
PREPARATION OF CEPHALOTHORAX 25
HYDROLYSIS OF CEPHALOTHORAX USING DIFFERENT PROCESSES 25
PROCESS DETAILS OF PROTEIN HYDROLYSATE HYDROLYSIS PROCESS 26
RATE OF HYDROLYSIS 27
ENZYMATICALLY HYDROLYSIS OF SEAFOOD WAST E TO YIELD PROTEIN
HYDROLYSATE 28
EXTRACTION OF FISH VISCERAL HYDROLYSATE 30
COLLECTION OF RAW MATERIALS AND PRETREAT MENT 30
HYDROLYSIS OF PRETREATED MATERIAL 31
ACID HYDROLYSIS 32
ALKALINE HYDROLYSIS 32
ENZYMATIC HYDROLYSIS 33
RECOVERY OF FPH 34
PRINCIPLES OF PLANT LAYOUT 35
STORAGE LAYOUT: 35
EQUIPMENT LAYOUT: 35
SAFETY: 37

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PLANT EXPANSION: 37
FLOOR SPACE: 37
UTILITIES SERVICING: 38
BUILDING: 38
MATERIAL-HANDLING EQUIPMENT: 39
RAILROADS AND ROADS: 40
MAJOR PROVISIONS IN ROAD PLANNING FOR MU LTIPURPOSE SERVICE A RE: 41
PLANT LOCATION FACTORS 42
PRIMARY FACTORS 42
1. RAW-MATERIAL SUPPLY: 42
2. MARKETS: 42
3. POWER AND FUEL SUPPLY: 42
4. WATER SUPPLY: 43
5. CLIMATE: 43
SPECIFIC FACTORS 44
6. TRANSPORTATION: 44
A. AVAILABILITY OF VARIOUS SERVICES AND PROJECTED RATES 44
7. WASTE DISPOSAL: 44
8. LABOR: 44
9. REGULATORY LAWS: 45
10. TAXES: 45
11. SITE CHARACTERISTICS: 45
12. COMMUNITY FACTORS: 46
13. VULNERABILITY TO WARTIME ATTACK: 46
14. FLOOD AND FIRE CONTROL: 46
EXPLANATION OF TERMS USED IN THE PROJECT REPORT 47
1. DEPRECIATION: 47
2. FIXED ASSETS: 47
3. WORKING CAPITAL: 47
4. BREAK-EVEN POINT: 47
5. OTHER FIXED EXPENSES: 48
6. MARGIN MONEY: 48
7. TOTAL LOAD: 48
8. LAND AREA/MAN POWER RATIO: 48
PROJECT IMPLEMENTATI ON SCHEDULES 49
INTRODUCTION 49
PROJECT HANDLING 50
PROJECT SCHEDULING 51

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PROJECT CONSTRUCTION SCHEDULE 51
TIME SCHEDULE 52
ADDRESSES OF RAW MAT ERIAL SUPPLIERS 53
SUPPLIERS OF SULPHURIC ACID 53
SUPPLIERS OF HYDROCHLORIC ACID 54
ADDRESSES OF PLANT & MACHINERY SUPPLIERS 55
SUPPLIERS OF HYDROLYZING TANK/NEUTRALIZER 56
SUPPLIERS OF DRYER 57
SUPPLIERS OF BOILER 58
SUPPLIERS OF CENTRIFUGE 59
SUPPLIERS OF STORAGE TANK 60
SUPPLIERS OF CRYSTALLIZER 61
SUPPLIERS OF EVAPORATOR 62
SUPPLIERS OF FILTER 63

APPENDIX – A :

01. PLANT ECONOMICS A-1
02. LAND & BUILDING A-2
03. PLANT AND MACHINERY A-3
04. OTHER FIXED ASSESTS A-4
05. FIXED CAPITAL A-5
06. RAW MATERIAL A-6
07. SALARY AND WAGES A-7
08. UTILITIES AND OVERHEADS A-8
09. TOTAL WORKING CAPITAL A-9
10. TOTAL CAPITAL INVESTMENT A-10
11. COST OF PRODUCTION A-11
12. TURN OVER/ANNUM A-12
13. BREAK EVEN POINT A-13
14. RESOURCES FOR FINANCE A-14
15. INSTALMENT PAYABLE IN 5 YEARS A-15
16. DEPRECIATION CHART FOR 5 YEARS A-16
17. PROFIT ANALYSIS FOR 5 YEARS A-17
18. PROJECTED BALANCE SHEET FOR (5 YEARS) A-18

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COST ESTIMATION

Plant Capacity 20 MT/Day

Land & Building (4000 sq.mt.) Rs. 2.88 Cr

Plant & Machinery Rs. 4.73 Cr

Working Capital for 2 Months Rs. 3.12 Cr

Total Capital Investment Rs. 11.30 Cr

Rate of Return 18%

Break Even Point 74%