Diapositivas para explicar un articulo cientifco

partes de una presentación de un articulo

1. Transplastomic tomatoes expressing double-stranded RNA against a conserved gene are efficiently protected from multiple spider mites ( Wu et al., 2022)

2. Study Design Species tested : Tetranychus evansi , T. truncatus , T. cinnabarinus Evaluate wheter transplastomic tomato plants expressing dsRNA targeting - actin can protect against multiple spider mite species Objetive Transformation by Biobalistic Agrotransformation Measured survival , reproduction and plant damage . Molecular confirmation : RT-PCR, northen blotting , southern blottingn and qPCR . Target: conserved - actin gene region of three spider mite species . Plastid transformation expected to accumulate more dsRNA .

3. Results New Phytologist, Volume: 237, Issue: 4, Pages: 1363-1373, First published: 03 November 2022, DOI: (10.1111/nph.18595) Fig. 2 Generation of transplastomic and nuclear transgenic tomato plants expressing double-stranded  ACT  gene fragments from spider mites. C onstruct (pMT18) C onstruct (pMT51)   Transplastomic plants     Nuclear- transformed plants   H omoplasmic status ( Southern blot analysis) L evels of ds ACT  by Northern blotting Lack of segregation Normal growth in transformed lines N uclear lines are less efficient at accumulating ds RNA

4. Results New Phytologist, Volume: 237, Issue: 4, Pages: 1363-1373, First published: 03 November 2022, DOI: (10.1111/nph.18595) Fig. 3 Feeding assays of  Tetranychus evansi  on wild-type, nuclear transgenic, and transplastomic tomato plants. Kaplan- Meier curve Suppression of  ACT   expression R esistance levels of transplastomic and nuclear transgenic plants to  T. evansi P rotective effects of dsRNA

5. Conclusions Plastid-mediated RNAi protects tomato plants from spider mites. Transplastomic plants more effective than nuclear. Expands RNAi application for sustainable crop protection