DNA extraction from Banana, green pea seeds and spinach.pptx

Isolation of DNA from onion, green pea seeds,papaya and banan

1. To isolate DNA from plant materials such as spinach, green peas, papaya and any other available plant material.

2. Necessary Materials & Apparatus Any available plant materials Mortar and pestle Test tubes

3. Beakers Chilled Ethanol

4. Spool Enzymes (Cellulase, ribonuclease, lipases, protease)

5. Procedure Take the available plant material and grind it in the mortar.

6. Treat the material with cellulase to break down the cell wall of the plant cells.

7. Next, treat it with protease to hydrolyze the peptide bonds of proteins in the plant material. In other words, the enzyme removes the histone proteins which are intertwined with the DNA.

8. Dissolve RNA with ribonuclease

9. Use lipase to dissolve lipids

10. Add chilled ethanol to enable the precipitation of the DNA. It essentially increases DNA concentration.

11. Use spooling to extract the precipitated DNA

12. Spooling involves winding the fine threads of DNA on to a reel.