Embryo culture and its application

This PowerPoint contains information related to embryo culture its basics, methods, procedure along with its application.

1. EMBRYO CULTURE AND ITS APPLICATION BY – DIVYA BADONI Bsc. (H) Biotechnology 2 nd year IMSUC, Ghaziabad

2. EMBRYO CULTURE Embryo culture is a type of Tissue Culture technique. WHAT IS EMBRYO ? Male gamete + Female gamete (fusion) fertilization zygote develop embryo

3. WHAT IS EMBRYO CULTURE ? Deals with sterile isolation and In- vitro growth of zygotic immature/mature embryo into a plantlet. In Somatic Embryogenesis technique we develop the embryo into the plant with the help of Explant. The part we select out of an living organism from which we make the whole plant is called as Explant.

4. METHODS OF EMBRYO CULTURE methods of embryo culture Mature embryo culture Immature embryo culture OR Embryo rescue method

5. MATURE EMBRYO CULTURE The development stage is gained by embryo. Isolation of mature embryo from ripe seeds. Used when embryo doesn’t survives in-vivo(because of seed dormancy chemical inhibitors or mechanical resistance).

6. IMMATURE EMBRYO CULTURE/ EMBRYO RESCUE METHOD It is still in the development stage. Isolation of immature embryo from unripe/hybrid/sterile seed which fails to develop. Used to rescue embryo of wide crosses or distant hybridization (inter-specific and inter-generic cross)

7. PROCEDURE OF EMBRYO CULTURE SEED SURFACE STERILIZATION ISOLATION OF EMBRYO EMBRYO KEPT IN NUTRIENT MEDIA (MS/BS/White media) simple media for mature and complex media with high concentration of sucrose for immature embryo organogenesis differentiation CALLUS PLANTLETS

8. APPLICATIONS OF EMBRYOCULTURE Production of plant from embryo. Study the relationship between embryo and endosperm. Prevents Embryo abortion . Overcome seed dormancy. Sterile seed due to early ripening e.g. Peach, cherry, apricot and plum. Used in scientific study as well Clonal propagation

9. Embryo endosperm transplant. Haploid production. Biotechnology. Shortening of breeding cycle.