Errat

Errat

1. ERRA T

2. Content Introduction Objectives Input & Output Methodology Results Analysis Conclusion

3. ERRAT is a web based tool that is utilized for protein model evaluation, either predicted model or experimentally determined and downloaded from Protein Data Bank. Introduction

4. “ ERRAT analyzes the statistics of non-bonded interactions between different atom types and plots the value of error functions.”

5. Objectives Research Outcome ERRAT is utilized for analysis of protein model of non-bonded residues & protein model evaluation. Available on ERRAT is a web based tool that can accessed through any web browser. Analyzes ERRAT is used for the backbone statistical analysis of the protein model.

6. Input The server requires to upload the file containing the target protein structure. The server results in plotting initial model and a final model. According to the analysis by ERRAT, the final model is significantly improved relative to the initial model. Output Input & Output

7. Comparison Compared the reported structure to a database of reliable high-resolution structures. Sensitivity The method is sensitive to smaller errors than 3-D Profile analysis Prediction The program verify protein structures determined by crystallography. Verification Verifying protein structures determined by crystallography. Error Function The Error function is based on the statistics of non-bonded atom-atom interactions. Methodology Error Values Error values are plotted as a function of the position of a sliding 9-residue window.

8. This is how ERRAT evaluates the particular model of protein. The graph represents three different regions for specific residues. The description for these regions are given in the table.

9. Regions Description 1st Green Highly Reliable Validated 2n d Yellow Not much Reliable/Warning 3rd Red Error Results Analysis

10. ERRAT (protein model evaluation tool) is sensitive to smaller errors than 3-D Profile analysis. Overall score >= 80 is considered as more than an accurately predicted model. Conclusion

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