Experimental animals used in pharmacology

This ppt gives information about experimental animals used in pharmacology practical

1. Experimental animals used in Pharmacology Prepared by – Mr. Digambar Jadhav Lecturer College of pharmacy, Paniv

2. List of experimental animals used in pharmacology Guinea pig Albino rat Albino mouse Rabbit Frog

3. 1. Guinea pig Introduction It is docile animal It is highly susceptible to TB & Anaphylaxis It is also sensitive to histamine & penicillin Experimental use 1. Evaluation of bronchodilator 2. Anaphylactic and immunological studies 3. Evaluation of local anaesthetics 4. Guinea pig ilium is the most common tissue preparation used to study spasmogens & antipasmodics

4. 1. Guinea pig Scientific name Cavia porcellus Body weight 500-800 gm Body temperature 38.3 Respiration rate 60-110 breaths/min Herat rate 250-300 beats/min Blood pressure -- Blood volume 65-90 ml/kg Life span 2 years Gestation period 67 days Breeding age 365 days Breeding life 1 year Parameters

5. 2. Albino rat Introduction Rats are small Rats are easy to breed & maintain Vomiting center is absent so drugs can be administered orally Various rat tissue used are colon, stomach, uterus, caecum & vas deferens Experimental use 1. Psychopharmacological studies 2. Study of analgesic & anticonvulsant drugs 3. Bioassay of various hormones such as insulin, oxytocin 4. Study of hepatotoxic & antihepatotoxic compounds

6. 2. Albino rat Scientific name Rattus norvegicus Body weight 200-300 gm Body temperature 37.5 Respiration rate 80-150 breaths/min Herat rate 260/450 beats/min Blood pressure 130/90 mm Hg Blood volume 50-65 ml/kg Life span 2-3 years Gestation period 21 days Breeding age 60-80 days Breeding life 1-1.25 years Parameters

7. 3. Albino mouse Introduction They are small, cheap & easy to handle. Experimental use 1. Toxicological studies 2. Study of bioassay of insulin 3. Study of analgesic & anticonvulsant drugs 4. Study of drugs acting on central nervous system 5. Study of chemotherapeutic agents

8. 3. Albino mouse Scientific name Mus musculus Body weight 25-30 gm Body temperature 37.4 Respiration rate 90-180 breaths/min Herat rate 300/750 beats/min Blood pressure 120/75 mmHg Blood volume 70-80 ml/kg Life span 1-2 years Gestation period 19 days Breeding age 42-56 days Breeding life 0.75-1.5 years Parameters

9. 4. Rabbit Introduction It is docile animal with large ear The enzyme atropine esterase is present in liver & plasma so it can tolerate large dose of belladonna. Experimental use 1. Pyrogen testing 2. Bioassay of antidiabetic drugs & sex hormones 3. Study of Mydriatics & M iotics drugs 4. Study of Local anaesthetics

10. 4. Rabbit Scientific name Oryctolagus cuniculus Body weight 2000-3000 gm Body temperature 38.3 Respiration rate 35-60 breaths/min Herat rate 205-308 beats/min Blood pressure - Blood volume 65-90 ml/kg Life span 4-5 years Gestation period 32 days Breeding age 90-120 days Breeding life 3 years Parameters

11. 5. Frog Introduction It is amphibian animal & safe to handle Isolated tissue preparation such as heart, rectus abdominus muscle are used Use of frogs for experimental purpose has been banned Experimental use 1. To study effect of drug on heart 2. Study of drugs acting on central nervous system 3. Study of drugs acting on neuromuscular junction