Eye donation

ophthalmology

1. EYE DONATION

2. INCIDENCE OF BLINDNESS

3. EYE PLEDGING Eyes should not be removed from a living person Eyes should not be purchased or sold Individuals can register in the nearest eye bank for donating their eyes after death. Eyes are collected after getting death information and consent from nearest relatives.

4. PLEDGE FORM Name : Gender : Date of Birth : Submit (mm/ dd / yyyy ) e-mail id : Address : Country : State : City : Pincode : Phone No (Residence) : Mobile : The information furnished above is correct to my knowledge I PLEDGE

5. WHO CAN DONATE Any age Any sex Any religion Even if not pledged while alive Spectacle wearers Undergone cataract or other eye surgeries Diabetics & hypertensives

6. WHO CANNOT HIV + HEPATITIS B , C Rabies Syphilis Creutzfeldt Jacob Disease Alzeimer’s Disease Septicemia Leukemia Death From Unknown Cause

7. Corneal diseases Intraocular tumours eg . Retinoblastoma,melanoma Iridocyclitis Endophthalmitis

8. DONOR REMOVAL

9. TIME OF REMOVAL : Eyes must be collected within 6 hours of death.

10. BEFORE REMOVAL dgg

11. INFORMATION NOTED AGE OF DONOR TIME OF DEATH CAUSE OF DEATH

12. Methods of REMOVAL ENUCLEATION

13. CORNEO-SCLERAL BUTTON EXCISION

14. 5 ml blood withdrawn from donor to screen for infectious diseases like HIV, Hepatitis

15. TRANSPORT TO EYE BANK MOIST CHAMBER METHOD Glass bottles with metal stand

16. EVALUATION OF DONOR CORNEA SLIT LAMP BIOMICROSCOPY SPECULAR MICROSCOPY- endothelial cell count

17. GRADING OF CORNEA

18. STORAGE SHORT TERM (2-4 days) INTERMEDIATE TERM ( 2 weeks) LONG-TERM (months-years)

19. SHORT TERM MOIST – CHAMBER METHOD (2 Days) McCAREY KAUFMAN MEDIUM (4days)

20. MOIST – CHAMBER METHOD The whole donor eye is kept in a sterile jar filled with saturated moist atmosphere at 4°C Upto 2 days Advantage – simplest, cheap, esp in developing countries. Disadvantage – intact eye, hence endothelium exposed to aqueous changes.

21. McCAREY KAUFMAN MEDIUM Described by McCarey and Kaufman Contents Tissue culture TC-199 5% dextran-40 HEPES buffer Gentamycin Pink in colour Upto 4 days. Only Corneoscleral button

22. INTERMEDIATE TERM Corneal storage media containing chondroitin sulfate Enhanced corneal dehydration. OPTISOL K-SOL DEXSOL Upto 2 weeks.

23. LONG TERM ORGAN – CULTURE METHOD Corneas are incubated in tissue culture medium supplemented with fetal calf serum, antibiotics, and antimycotics at 30–37°C. Upto 30 days CRYOPRESERVATION: Freezing the donor cornea stored in liquid nitrogen. Upto 1 year

24. UTILIZATION OF DONOR CORNEA KERATOPLASTY Penetrating – full thickness graft Lamellar – partial thickness graft Optical – to restore vision Therapeutic – remove infected tissues in non-healing ulcers Tectonic – restore integrity of eyeball Cosmetic