Fate map

The ppt gives general overview of-
Introduction
Mapping technique
1.Natural marking
2.Artificial marking
a. Vital staining technique
b. Carbon particle marking technique
c. Radioactive marker technique
3.Genetic marking
Uses

1. FATE- MAP

2. CONTENT Introduction Mapping technique 1.Natural marking 2.Artificial marking a. Vital staining technique b. Carbon particle marking technique c. Radioactive marker technique 3.Genetic marking Uses

3. FATE MAP Def. Map that shows fate of every region of blastula under normal developmental conditions .

4. MAPPING TECHNIQUES Natural marking Artificial marking Genetic marking

5. NATURAL MARKING Cytoplasm of fertilized egg has natural colour differences in it’s various regions. Ex. Ascidian– Styela partita

6. ARTIFICIAL MARKING 1.Vital staining technique 2. Carbon particle marking technique 3.Radioactive marker technique

7. 1.Vital staining technique Use -Vital stains like Nile blue sulphate , Neutral red , Janus green , Bismark brown.

8. Advantages- Stain retained for considerable time. Doesn’t interfere with cell processes. Only those cells receiving the dye retain dye; doesn’t diffuse into the neighbouring cells. Use -Tiny particles of carbon or other cell surface adhering particles like chalk. 2.Carbon Particle marking method

9. Use - Radioactive markers like Tritiated thymidine 3.Radioactive labelling methods Ex . Fate mapping Zebrafish using a fluorescent dye.

10. GENETIC MARKING Mosaic/ chimeric embryo is created. 1.The quail cell have heterochromatin. 2.Cell specific antigens that are quail specific . Chick Quail

11. USES Used in following the mechanism of gastrulation. Helpful in following the movement and ultimate position of tissues.