Fluorometry- BP701T. INSTRUMENTAL METHODS OF ANALYSIS

Fluorimetry ( BP701T. INSTRUMENTAL METHODS OF ANALYSIS (Theory)

Theory, Concepts of singlet, doublet and triplet electronic states, internal and external
conversions, factors affecting fluorescence, quenching, instrumentation and
applications

1. FLUOROMETRY BY- Mr. Rahul Assistant Professor - Corporate Institute of Pharmacy Bhopal

2. We will see.

3. INTRODUCTION The term comes from "fluorescence," which refers to the emission of light by a substance that has absorbed light, and "- metry ," meaning measurement. So, fluorometry is essentially the measurement of fluorescence.

4. DEFINATION Fluorometry is a technique used to measure the fluorescence (or light) emitted by a substance when it is exposed to light. It helps in identifying and quantifying different substances based on their fluorescent properties.

5. PRINCIPLE

6. ABSORPTION

7. EXCITATION

8. Relaxation and Emission :

9. DETACTION

10. INSTRUMENTATION

11. KEY COMPONENTS

12. SOURCE OF LIGHT

13. MURCURY ARC LAMP     TUNGSTEN LAMP      XENON ARC LAMP

14. FILTERS

15. SAMPLE HOLDERS

16. DETACTORS

17. FACTORS AFFECTING

18. NATURE OF MOLECULES

19. CONCENTRATION

20. ADSORPTION

21. OXYGEN & pH