Gel doc system

gel documentation system.........

1. Gel Documentation Aamir.A.Palwala M.Sc. Microbiology Semester-II Department of Biosciences Saurashtra University, Rajkot

2. Gel documentation , also known as gel doc. system , gel image system , or gel imager. It is widely used in molecular biology laboratories for imaging and documentation of nucleic acid and protein polyacrylamide or agarose gels typically stained with ethidium bromide or flurophores such as SYBR green. Generally , a gel doc. is composed of ultraviolet ( UV ) light transilluminator , a hood to shield external light sources and CCTV camera for image capturing.   INTRODUCTION

3. PRINCIPLE Principle of fluorescence with fluorescent staining of nucleic acids , a fluorescent substance that has bound to nucleic acid is exited by ultraviolet irradiation and emits fluorescent light. Ethidium bromide binds specifically to nucleic acid and the amount of bonding depends on the molecular weight and concentration of the nucleic acid. In other words , a band for a large amount will shine brighter ; conversely , fluorescence will be weaker for a band for a small amount.  

4. PURPOSE AND ANALYSIS Photography of stained gel. Print out of photographic data. Image data is displayed in real time. Image still displayed can be printed out with a video printer or saved to a compact flash media for future use.  

5. components of gel doc. System   Camera Lenses Filters Overhead illuminator   Visible light Adjustable stage   Darkroom Tr ansilluminator  

6. components of gel doc. System   Camera : Ultraviolet camera ranging from 1.4m upto 8.3m pixel. Lenses : All lanses are computer controlled and motor driven. Here lances automatically tracks upwards or downwards movements of sample using auto focus. Filters : There is an extensive range of emission filters use for an array of applications. Over head illuminator : White light , Ultra violet light , and LED lighting option Visible light : For extending the transmitted light application. Visible light convert screen for visible light application.  

7. components of gel doc. System Adjustable stage : This enables samples to be moved closer to the camera. Darkroom : dark room is suitable for advanced chemiluminescence , fluorescence and visible light application. An electronic auto-door lock with security function prevents interruption to long exposures. Transilluminator : It has a variable intensity setting and has a safety cut-off.

8. QUANTITY ONE FROM BIORED   It is a software which is required to operate gel doc. system. It is user friendly version windows based option. It has a various tool for image capturing and transformation. There is a separate tool for identifying , detecting and drawing lense and bands. Band attributes include band creation , GAUSS model bands , density of a band , band boundries etc…… There is a separate function for storing standard data , standard curve for known molecular weight and size. A separate volume analysis tool for volume contour , free hand , rectangle , circle and array. The software also has options for VNTR display , VNTR calculation , differential display and colony counting applictions . The software can generate reports for individual bands , all lenses , band types , 1-dimension analysis , lane compaing , phylogenetic tree and other simmilarity matrix options.  

9. application 1-D Electrophoresis Documentation 2-D Documentation Protein Gel Documentation Blotting technique analysis