Gram positive and Gram negetive Bacteria

About Gram positive and Gram negative bacteria

1. WELCOME

2. PRESENTATION ON Gram positive Bacteria & Gram negative Bacteria

3. Submitted To MD. EZAZUL HAQUE Lecturer Department of pharmacy UODA

4. Submitted By Silvia Shomba ID. No. – 031153004 Batch – 46 th Department Of Pharmacy APRIL 9, 2015

5. Name of Topics What is Gram positive Bacteria? What is Gram negative Bacteria? Differences Between Gram positive & Gram negative Bacteria. What is Gram staining? How to perform a Gram staining procedure?

6. Definition Of Gram P ositive Bacteria & Gram Negative Bacteria Gram Positive Bacteria Positive result Crystal violet stain Gram Negative Bacteria Negative result Pink or red color

7. Differences Between Gram positive & Gram negative Bacteria Gram reaction Cell wall Outer membrane Glycocalyx Peptidoglycan layer Porins Lipopolysaccharide Content (LPS) Lipid & lipoprotein content Toxin Produced

8. Gram Staining Common Technique Differentiate two large group Distinguishes by coloring

9. Procedure Of Gram Staining Preparation & Fixation of smear Primary Staining Application of Iodine Alcohol wash Application of Safranin

10. THANK YOU