Gram staining & structural Staining

It includes Gram staining, endospore staining, flagella staining.

1. GRAM STAINING & STRUCTURAL STAINING Guided By: Dr. N. S. Devaki Course Coordinator Dept. of Molecular Biology Presented By: Inchara R 4 th Semester Molecular Biology 22/03/2019

2. CONTENTS Introduction Gram Staining Endospore Staining Flagella Staining Conclusion References Acknowledgement

3. INTRODUCTION Fig 01: Conversion of Benzene into a Stain

4. Fig 02 : Crystal Violet structure

5. GRAM STAINING Fig 03: Steps involved in Gram Staining

6. Fig 04: Lactobacillus Gram positive bacteria Fig 05: Citrobacter freundii Gram negative bacteria

7. ENDOSPORE STAINING Fig 06: Steps involved in Endospore Staining

8. Fig 07: Bacillus subtilis Fig 08: Endospore stain of B acillus species

9. Fig 08: Bacillus cereus with Peritrichous flagella Fig 09: Vibrio cholerae with M onotichous flagella Flagella Staining

10. Fig 10: Lophotrichous Flagella Transmission electron micrograph Fig 11: Peritrichous flagella of Proteus vulgaris Light microscope

11. CONCLUSION

12. REFERENCES Prescott, Harley and Klein’s Microbiology. 2008. Joanne M. Willey, Linda M. Sherwood, Christopher J. Woolverton, McGraw hill education, North America, 1088pp. http :// www.biologydiscussion.com/bacteria/staining-procedures-for-detecting-bacteria/55104   https://microbeonline.com/types-of-staining-techniques-used-in-microbiology-and-their-applications /

13. ACKNOWLEDGEMENT I would like to thank the dept. of Molecular Biology for providing this opportunity to present this seminar . I would also like to thank my guide DR. N.S. Devaki ma’am for her valuable guidance. Thank you one and all. 