GROUP-B.-SEED-GERMINATION-LIGHT-and-DARK (1).pptx

Science

1. Light and Dark Seed Germination of Pechay Brassica rapa subsp. Chinensis and Lettuce Lactuca sativa (CROP SCIENCE 1) GROUP 4 Members: Aquino, Erika H. Consorte , Feona C. Eugenio, Anfie G. Hernandez, Angelyn V. Hinagpisan , Melvin M. Pajarillo , Bernadette C. Panahon , Gelcyfer P. Rubia, Raymond P.

2. Introduction Seed germination is the most important stage in a plants life cycle. Water, air, temperature and light are all essential for the seed germination process starting from imbibition, activation and succeeding manifestation . Seed germination is a crucial process that influences crop yield and quality. Therefore, understanding the molecular aspects of seed dormancy and germination is of a great significance for the improvement of crop yield and quality . Seed germination is defined as the sum of events that begin with hydration of the seed and culminate in emergence of the embryonic axis (usually the radicle) from the seed coat.

3. Objectives At the end of this laboratory , the student will be able to: Demonstrate the activity effectively and clearly. Access and observe the process of germination period of a given seeds (Lettuce and Pechay ) in terms of the different area it was place. Discuss and compare the result of germination rate of the two different shades.

4. MATERIALS

5. 4 petri dishes Tissue paper 1 cardboard box sprayer w/ water 50 pcs. Seeds of pechay 50 pcs. seeds of lettuce

6. PROCEDURE: 1. Prepare 4 petri dishes lined with moist tissue paper. 2. Evenly distribute 50 seeds of lettuce in each of two petri dishes, and 50 seeds of pechay in each of the remaining petri dishes. 3. Label the petri dishes as lettuce in light, lettuce in darkness, pechay in light, pechay in darkness. 4. Place the two petri dishes in a well-lighted place, and the other two under a cardboard box. 5. Observe the set-up after one week and determine the germination rate of the seeds. 6. Establish whether the seeds were dominant or not and discuss how it was affected by light.

7. GERMINATION RATE OF LETTUCE (DARKNESS) Survival seedlings - 28 Total seeds - 50 28/50*100 = 56% GERMINATION RATE OF PECHAY (DARKNESS ) Survival seedlings- 38 Total seeds - 50 38/50*100 = 76%

8. GERMINATION RATE OF pechay (LIGHT) Survival seedlings- 42 Total seeds - 50 42/50*100 = 84% GERMINATION RATE OF LETTUCE (LIGHT) Survival seedlings - 39 Total seeds - 50 39/50*100 = 78%

9. Documentation Germination of Pechay and Lettuce (Light) Day #1 Day #2 Day #3 This period, sprouting of small tiny This day, we will see the gradual It continues to progress and show up w hite root. r emoval of seed coat. a tiny yellow green leaves.

10. Day #4 Day #5 Day #6 In this period, we will see the leaves are gradually opening. In this day, we observed the sprouting of dark green leaves. It continues to progress and growth of roots and leaves will happen.

11. Germination of Pechay and Lettuce (Darkness) Day #1 Day#3 Day #6 This period, sprouting of small tiny This day, we will see the gradual It continues to progress and white root. removal of seed coat . And we will a tiny yellow green leaves . see a tiny yellow leaves. Also, we will see a very thin stem

12. CONCLUSION It is safe to say that the presence of light tends to inhibit the growth of Pechay and Lettuce. According to the results on our activity, the germination of the Pechay and Lettuce seeds in expose to light are more likely to be more effective than the pechay and lettuce seeds in dark.