Hairy root culture

Hairy Root Cell Culture Seminar Paper Power Point Presentation by Abu Raihan

1. Hairy Root Cell Culture Presented by Abu Raihan M.Tech , 1 st year,Biotechnology

2. Culture Types Callus Culture Cell Suspension Culture Protoplast Culture Hairy Root Culture

3. Hairy Root Culture Definition: It is the culture produced after the infection of explants and cultures by the gram negetive Soil bacterium Agrobacterium rhizogenes . This processes take advantage of the naturally occuring hairy root disease in Dicotyledons .

4. Properties of hairy roots Genotype and phenotype stability Autotrophy in plant hormones Fast growth High levels of secondary metabolite production

5. Production of hairy root in plant cell Agrobacterium recognizes some signal molecules exuded by wonded plant cells and becomes attached to it. The bacteria contain the Root inducing plasmid ( Ri -plasmid). The bacteria Genetically trasfer part of the Ri -plasmid called the trasfer DNA (T-DNA ) to the plant genome .

6. Production of hairy root in plant cell The plant cell to proliferate by increasing the rate of cell division ( cytokine expression ) and cell elongation ( auxin expression) to produce the hairy roots. Produce the opines which is a type of unusual amino acids ( octopine , agropine , nopaline , mannopine amd cucumopine ) which is used by the bacterium as a carbon , nitrogen and energy source.

7. The Process

8. Ri -plasmid

9. Induction of hairy root cultures In Vitro Explants are wounded and then inoculated with Agrobacterium rhizogenes . Usually two or three days later, the explant can be transferred into solid media with antibiotics, such as cefotaxime , vancomycine or ampicillin to kill or eliminate redundant bacteria. The hairy roots will be induced within a short period of time, which varies from one week to over a month depending on different plant species. The decontaminated hairy roots can be subcultured on phytohormone –free medium.

10. Bioreactors for hairy root cultures Stirred tank bioreactor Air lift bioreactor Bubble column bioreactor Turbine blade bioreactor Trickle bed bioreactor Rotating drum bioreactor Spin filter bioreactor

11. Bubble column with hairy root culture picture

12. Agar plate with hair root picture

13. Hairy root cell culture picture

14. Advantages of hairy root cultures The hairy root system is genetically and biosynthetically stable. High production of secondary metabolites The culture can grow under phyto -hormone- free conditions. The culture shows fast growth which reduces the culture time and easy the handling.

15. Application of Hairy Root Cultures Functional analysis of genes. Expression foreign proteins. Production of secondary metabolites. The culture may produce compounds which is not found untransformed roots. The culture may change the composition of metabolites. The culture can be used to regenerate a whole plant.

16. Thanks