HFR cells are formed from homologous recombination due to integratio.pdf

HFR cells are formed from homologous recombination due to integration of the conjugate
plasmid into bacterial chromosome. Hfr cells may donate genes from its chromosomes to another
cell. Hfr cells do not possess a complete \"circular chromosome\" i.e. plasmid as F+ cell.
Therefore, the major events of Hfr (high frequency recombinant) cells contain F factor in its
chromosomal DNA often referred as \"episome\". F+ cell possess F factor known as plasmid & it
can donate to another cell through conjugation. Both Hfr & F+ cells are competent for
conjugation through sex pili or mating bridge & finally transfer F factor into the recipient cell.
Interrupted mating:
F factor of HFR cells is mainly pertaining to a class of conjugate plasmids which meticulously
originated to control sexual functions in prokaryotes via fertility inhibition. The origin of F-
factor is mainly with most common functional segments such as gene traJ, Ori, OriC. This
structure is going to promote high frequency recombination through interrupted mating into the
recipient cell. The structure of this F factor contains OriT for origin of transfer and acts as a
starting point for gene transmission to the bacterium of F- (in which no F factor). A streptomycin
antibiotic containing medium is used to kill the remaining Hfr donor cells after interrupting
conjugation. This is completely based on the streptomycin-sensitivity allele (str-s) in the Hfr
strains.
On the otherhand, streptomycin-resistance allele (str-r) presence in the recipient is employed
considerably to specifically destroy the Hfr donor cells after conjugation.
Solution
HFR cells are formed from homologous recombination due to integration of the conjugate
plasmid into bacterial chromosome. Hfr cells may donate genes from its chromosomes to another
cell. Hfr cells do not possess a complete \"circular chromosome\" i.e. plasmid as F+ cell.
Therefore, the major events of Hfr (high frequency recombinant) cells contain F factor in its
chromosomal DNA often referred as \"episome\". F+ cell possess F factor known as plasmid & it
can donate to another cell through conjugation. Both Hfr & F+ cells are competent for
conjugation through sex pili or mating bridge & finally transfer F factor into the recipient cell.
Interrupted mating:
F factor of HFR cells is mainly pertaining to a class of conjugate plasmids which meticulously
originated to control sexual functions in prokaryotes via fertility inhibition. The origin of F-
factor is mainly with most common functional segments such as gene traJ, Ori, OriC. This
structure is going to promote high frequency recombination through interrupted mating into the
recipient cell. The structure of this F factor contains OriT for origin of transfer and acts as a
starting point for gene transmission to the bacterium of F- (in which no F factor). A streptomycin
antibiotic containing medium is used to kill the remaining Hfr donor cells after interrupting
conjugation. This is completely based on the st.

1. HFR cells are formed from homologous recombination due to integration of the conjugate
plasmid into bacterial chromosome. Hfr cells may donate genes from its chromosomes to another
cell. Hfr cells do not possess a complete \"circular chromosome\" i.e. plasmid as F+ cell.
Therefore, the major events of Hfr (high frequency recombinant) cells contain F factor in its
chromosomal DNA often referred as \"episome\". F+ cell possess F factor known as plasmid & it
can donate to another cell through conjugation. Both Hfr & F+ cells are competent for
conjugation through sex pili or mating bridge & finally transfer F factor into the recipient cell.
Interrupted mating:
F factor of HFR cells is mainly pertaining to a class of conjugate plasmids which meticulously
originated to control sexual functions in prokaryotes via fertility inhibition. The origin of F-
factor is mainly with most common functional segments such as gene traJ, Ori, OriC. This
structure is going to promote high frequency recombination through interrupted mating into the
recipient cell. The structure of this F factor contains OriT for origin of transfer and acts as a
starting point for gene transmission to the bacterium of F- (in which no F factor). A streptomycin
antibiotic containing medium is used to kill the remaining Hfr donor cells after interrupting
conjugation. This is completely based on the streptomycin-sensitivity allele (str-s) in the Hfr
strains.
On the otherhand, streptomycin-resistance allele (str-r) presence in the recipient is employed
considerably to specifically destroy the Hfr donor cells after conjugation.

Solution

HFR cells are formed from homologous recombination due to integration of the conjugate
plasmid into bacterial chromosome. Hfr cells may donate genes from its chromosomes to another
cell. Hfr cells do not possess a complete \"circular chromosome\" i.e. plasmid as F+ cell.
Therefore, the major events of Hfr (high frequency recombinant) cells contain F factor in its
chromosomal DNA often referred as \"episome\". F+ cell possess F factor known as plasmid & it
can donate to another cell through conjugation. Both Hfr & F+ cells are competent for
conjugation through sex pili or mating bridge & finally transfer F factor into the recipient cell.
Interrupted mating:
F factor of HFR cells is mainly pertaining to a class of conjugate plasmids which meticulously
originated to control sexual functions in prokaryotes via fertility inhibition. The origin of F-
factor is mainly with most common functional segments such as gene traJ, Ori, OriC. This
structure is going to promote high frequency recombination through interrupted mating into the
recipient cell. The structure of this F factor contains OriT for origin of transfer and acts as a

2. starting point for gene transmission to the bacterium of F- (in which no F factor). A streptomycin
antibiotic containing medium is used to kill the remaining Hfr donor cells after interrupting
conjugation. This is completely based on the streptomycin-sensitivity allele (str-s) in the Hfr
strains.
On the otherhand, streptomycin-resistance allele (str-r) presence in the recipient is employed
considerably to specifically destroy the Hfr donor cells after conjugation.