High performance liquid chromatography(HPLC)

High-performance liquid chromatography (HPLC; formerly referred to as high-pressure liquid chromatography), is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture.

1. High performance liquid chromatography(HPLC) Presented by: Md. Faysal Ahmed Id : 151-29-760

2. Introduction: HPLC stands for “High-performance liquid chromatography ”(sometimes referred to as High-pressure liquid chromatography ). It is used in biochemistry and analytical chemistry to identify , quantify and purify the individual components of a mixture.

3. Principle of HPLC: High performance liquid chromatography (HPLC) is basically a highly improved form of column liquid chromatography to separate compounds that are dissolved in solution. High performance liquid chromatography (HPLC ) is principle is based on adsorption as well as partition chromatography is depending on the nature of stationary phase. HPLC is a chromatographic technique that can separate a mixture of compounds .

4. Applications of HPLC Pharmaceutical field Chemical & Petrochemical industry Forensic Biochemical separations Food analysis Qualitative analysis : Checking the purity of a compound Quantitative Analysis: Direct comparison method Calibration curve method

5. Advantages of HPLC High sensitivity High performance Rapid process and hence time saving Can be used for qualitative as well as quantitative estimation. Separations fast and efficient (high resolution power) Accurate quantitative measurements.